Evidence map›Paper›PMID 36012208›Full record

ArticleInternational journal of molecular sciences2022

Mitogen-Activated Protein Kinases Associated Sites of Tobacco Repression of Shoot Growth Regulates Its Localization in Plant Cells.

Luyao Wang, Ying Gui, Bingye Yang, Wenpan Dong, Peiling Xu, Fangjie Si, Wei Yang, Yuming Luo, Jianhua Guo, Dongdong Niu and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.1field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Luyao WangDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Ying GuiDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Bingye YangDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Wenpan DongDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Peiling XuDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Fangjie SiDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Wei YangJiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, Huaiyin Normal University, Huai'an 223300, China.
Yuming LuoJiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, Huaiyin Normal University, Huai'an 223300, China.
Jianhua GuoDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Dongdong NiuDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.ORCID 0000-0002-9926-0923
Chunhao JiangDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.ORCID 0000-0001-8826-9597
Nanjing Agricultural University · CNHuaiyin Normal University · CN

Funding

China Agriculture Research System of MOF and MARA CARS-21National Natural Science Foundation of China 31900303, 31701829, 31972322, 32102281The Opening Project of Key Construction Laboratory of Probiotics in Jiangsu Province JSYSZJ2019003The Science and Technology Project of Jiangsu Province BE2021364, BE2020408
6 · The paper itself

Abstract

Plant defense and growth rely on multiple transcriptional factors (TFs). Repression of shoot growth (RSG) is a TF belonging to a bZIP family in tobacco, known to be involved in plant gibberellin feedback regulation by inducing the expression of key genes. The tobacco calcium-dependent protein kinase CDPK1 was reported to interact with RSG and manipulate its intracellular localization by phosphorylating Ser-114 of RSG previously. Here, we identified tobacco mitogen-activated protein kinase 3 (NtMPK3) as an RSG-interacting protein kinase. Moreover, the mutation of the predicted MAPK-associated phosphorylation site of RSG (Thr-30, Ser-74, and Thr-135) significantly altered the intracellular localization of the NtMPK3-RSG interaction complex. Nuclear transport of RSG and its amino acid mutants (T30A and S74A) were observed after being treated with plant defense elicitor peptide flg22 within 5 min, and the two mutated RSG swiftly re-localized in tobacco cytoplasm within 30 min. In addition, triple-point mutation of RSG (T30A/S74A/T135A) mimics constant unphosphorylated status, and is predominantly localized in tobacco cytoplasm. RSG (T30A/S74A/T135A) showed no re-localization effect under the treatments of flg22,

Indexed as

NicotianaPlant CellsGibberellinsMitogen-Activated Protein KinasesPhosphorylationPlant ProteinsGibberellinsMitogen-Activated Protein KinasesPlant Proteinsabiotic stressMAPKphosphorylationprotein interactionRSG

Identifiers

PMID36012208
PMCPMC9409217
OpenAlexW4291016734

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.