Evidence map›Paper›PMID 36012126›Full record

ArticleInternational journal of molecular sciences2022

Extraction and Biological Evaluation of Matrix-Bound Nanovesicles (MBVs) from High-Hydrostatic Pressure-Decellularized Tissues.

Mako Kobayashi, Naoki Ishida, Yoshihide Hashimoto, Jun Negishi, Hideki Saga, Yoshihiro Sasaki, Kazunari Akiyoshi, Tsuyoshi Kimura, Akio Kishida

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.3field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 1 country.

Mako KobayashiInstitute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku 101-0062, Japan.ORCID 0000-0002-2413-5810
Naoki IshidaInstitute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku 101-0062, Japan.
Yoshihide HashimotoInstitute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku 101-0062, Japan.ORCID 0000-0003-0537-8291
Jun NegishiDepartment of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, 3-15-1 Tokida, Ueda 386-8567, Japan.
Hideki SagaKM Biologics Co., Ltd., 1314-1 Kyokushi Kawabe, Kikuchi-shi 869-1298, Japan.
Yoshihiro SasakiDepartment of Polymer Chemistry, Graduate School of Engineering, A3-317, Kyoto University, Katsura, Nishikyo-ku 615-8510, Japan.
Kazunari AkiyoshiDepartment of Polymer Chemistry, Graduate School of Engineering, A3-317, Kyoto University, Katsura, Nishikyo-ku 615-8510, Japan.
Tsuyoshi KimuraInstitute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku 101-0062, Japan.ORCID 0000-0003-0476-0624
Akio KishidaInstitute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku 101-0062, Japan.ORCID 0000-0002-2434-3564
Tokyo Medical and Dental University · JPKyoto University · JPKM Biologics (Japan) · JPShinshu University · JP

Funding

Japan Society for the Promotion of Science 21H04954, 19H04465
6 · The paper itself

Abstract

Decellularized tissues are widely used as promising materials in tissue engineering and regenerative medicine. Research on the microstructure and components of the extracellular matrix (ECM) was conducted to improve the current understanding of decellularized tissue functionality. The presence of matrix-bound nanovesicles (MBVs) embedded within the ECM was recently reported. Results of a previous experimental investigation revealed that decellularized tissues prepared using high hydrostatic pressure (HHP) exhibited good in vivo performance. In the current study, according to the hypothesis that MBVs are one of the functional components in HHP-decellularized tissue, we investigated the extraction of MBVs and the associated effects on vascular endothelial cells. Using nanoparticle tracking assay (NTA), transmission electron microscopy (TEM), and RNA analysis, nanosized (100-300 nm) and membranous particles containing small RNA were detected in MBVs derived from HHP-decellularized small intestinal submucosa (SIS), urinary bladder matrix (UBM), and liver. To evaluate the effect on the growth of vascular endothelial cells, which are important in the tissue regeneration process, isolated SIS-derived MBVs were exposed to vascular endothelial cells to induce cell proliferation. These results indicate that MBVs can be extracted from HHP-decellularized tissues and may play a significant role in tissue remodeling.

Indexed as

Endothelial CellsTissue EngineeringExtracellular MatrixHydrostatic PressureRNATissue ScaffoldsRNAdecellularized tissuehigh-hydrostatic pressurematrix-bound nanovesicles

Identifiers

PMID36012126
PMCPMC9407827
OpenAlexW4291172289

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.