Evidence map›Paper›PMID 36011310›Full record

ArticleGenes2022

Qualitative and Quantitative Detection of Mealworm DNA in Raw and Commercial Food Products Using Real-Time PCR.

Małgorzata Natonek-Wiśniewska, Piotr Krzyścin, Anna Koseniuk

Abstract readCase Reports
In one paragraph

Article in Genes, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Małgorzata Natonek-WiśniewskaDepartment of Animal Molecular Biology, National Research Institute of Animal Production, 1, Krakowska Street, 32-083 Balice, Poland.ORCID 0000-0003-2132-2829
Piotr KrzyścinDepartment of Animal Molecular Biology, National Research Institute of Animal Production, 1, Krakowska Street, 32-083 Balice, Poland.ORCID 0000-0002-2254-0388
Anna KoseniukDepartment of Animal Molecular Biology, National Research Institute of Animal Production, 1, Krakowska Street, 32-083 Balice, Poland.ORCID 0000-0002-7236-567X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Considering food safety and an increasing public awareness of the ingredients, production process and origin of foods, the application of insects as food requires the development of tests for the reliable identification of their presence. The aim of the study was (1) the determination of appropriate modifications of the selected method for isolating the DNA of two life stages of mealworm, i.e., larva and adult, from commercial food products; (2) the determination of the method parameters for the qualitative and quantitative analysis of mealworm contents based on the detection of a species-specific mitochondrial DNA fragment, using real-time PCR; (3) the application of a method to test the commercial food products of mealworm. A total of nine species of adult insect were investigated (field cricket, Dubia cockroach, Madagascar cockroach, banded cricket, migratory locust, yellow mealworm, superworm, house fly and lacewing), theirlarvaes (yellow mealworms and superworms) and thirteen commercial food products (dried whole insects, powder and granules) representing various insect species and origins which were purchased from the European market. The obtained results showed that the efficiency of the modification of the DNA extraction method is dependent on the life stage of the mealworm. We proved the high sensitivity of the test, with the range of the method being 0.1-100%; we also proved the biological specificity in this range, and the linearity. The linearity of the test was also statistically verified using the Fisher-Snedecor test. One-way variance analysis showed statistically significant differences between the c

Indexed as

TenebrioAnimalsDNAInsectaLarvaReal-Time Polymerase Chain ReactionDNAinsectsmealwormmtDNAspecies identification

Identifiers

PMID36011310
PMCPMC9407359

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.