Evidence map›Paper›PMID 36010659›Full record

ArticleCells2022

Cellular Therapy Using Epitope-Imprinted Composite Nanoparticles to Remove α-Synuclein from an In Vitro Model.

Mei-Hwa Lee, Jeng-Shiung Jan, James L Thomas, Yuan-Pin Shih, Jin-An Li, Chien-Yu Lin, Tooru Ooya, Lilla Barna, Mária Mészáros, András Harazin and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Mei-Hwa LeeDepartment of Materials Science and Engineering, I-Shou University, Kaohsiung 84001, Taiwan.
Jeng-Shiung JanDepartment of Chemical Engineering, National Cheng Kung University, Tainan 70101, Taiwan.ORCID 0000-0002-8379-404X
James L ThomasDepartment of Physics and Astronomy, University of New Mexico, Albuquerque, NM 87131, USA.
Yuan-Pin ShihDepartment of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung 81148, Taiwan.
Jin-An LiDepartment of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung 81148, Taiwan.
Chien-Yu LinDepartment of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung 81148, Taiwan.
Tooru OoyaGraduate School of Engineering, Department of Chemical Science and Engineering, Kobe University, Kobe 657-8501, Japan.ORCID 0000-0001-7766-5455
Lilla BarnaInstitute of Biophysics, Biological Research Centre, H-6726 Szeged, Hungary.ORCID 0000-0002-6040-8513
Mária MészárosInstitute of Biophysics, Biological Research Centre, H-6726 Szeged, Hungary.ORCID 0000-0002-4847-9618
András HarazinInstitute of Biophysics, Biological Research Centre, H-6726 Szeged, Hungary.ORCID 0000-0002-0904-5606
Gergő PorkolábInstitute of Biophysics, Biological Research Centre, H-6726 Szeged, Hungary.ORCID 0000-0002-2495-0479
Szilvia VeszelkaInstitute of Biophysics, Biological Research Centre, H-6726 Szeged, Hungary.ORCID 0000-0001-8864-1184
Maria A DeliInstitute of Biophysics, Biological Research Centre, H-6726 Szeged, Hungary.ORCID 0000-0001-6084-6524
Hung-Yin LinDepartment of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung 81148, Taiwan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Several degenerative disorders of the central nervous system, including Parkinson's disease (PD), are related to the pathological aggregation of proteins. Antibodies against toxic disease proteins, such as α-synuclein (SNCA), are therefore being developed as possible therapeutics. In this work, one peptide (YVGSKTKEGVVHGVA) from SNCA was used as the epitope to construct magnetic molecularly imprinted composite nanoparticles (MMIPs). These composite nanoparticles were characterized by dynamic light scattering (DLS), high-performance liquid chromatography (HPLC), isothermal titration calorimetry (ITC), Brunauer-Emmett-Teller (BET) analysis, and superconducting quantum interference device (SQUID) analysis. Finally, the viability of brain endothelial cells that were treated with MMIPs was measured, and the extraction of SNCA from CRISPR/dCas9a-activated HEK293T cells from the in vitro model system was demonstrated for the therapeutic application of MMIPs.

Indexed as

Molecular ImprintingNanoparticlesalpha-SynucleinEndothelial CellsEpitopesHEK293 CellsHumansalpha-SynucleinEpitopesgene activationmagnetic nanoparticlespeptide imprintingprotein extractionα-synuclein

Identifiers

PMID36010659
PMCPMC9406856

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.