Evidence map›Paper›PMID 36009743›Full record

ArticleAnimals : an open access journal from MDPI2022

Evaluation of

Violeta Igna, Ada Telea, Tiana Florea, Roxana Popp, Adrian Grozea

Open access · goldAbstract read
In one paragraph

Article in Animals : an open access journal from MDPI, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.5field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 11 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Violeta IgnaFaculty of Veterinary Medicine, Banat's University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara, 300645 Timisoara, Romania.ORCID 0000-0002-7314-4641
Ada TeleaCenter for Gene and Cellular Therapies in the Treatment of Cancer-OncoGen, Clinical Emergency County Hospital "Pius Brinzeu" Timisoara, 300723 Timisoara, Romania.
Tiana FloreaFaculty of Veterinary Medicine, Banat's University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara, 300645 Timisoara, Romania.
Roxana PoppFaculty of Veterinary Medicine, Banat's University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara, 300645 Timisoara, Romania.
Adrian GrozeaFaculty of Bioengineering of Animal Resources, Banat's University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara, 300645 Timisoara, Romania.ORCID 0000-0002-7978-5247
Univesity of Life Science "King Mihai I" from Timisoara · ROSpitalul Clinic Judeţean de Urgenţă "Pius Brînzeu" Timişoara · RO

Funding

Executive Agency for Higher Education, Research, Development and Innovation Funding 182/2014
6 · The paper itself

Abstract

The alarming decline in sturgeon populations doubled by growing demands for sturgeon products, urge us to prevent the loss of these species. Fish stocking in natural habitats and developing fish farms are viable solutions, which can be successfully implemented with the help of reproductive biotechnologies. Despite the fact that semen cryopreservation is admittedly an important step for saving the Russian sturgeon, a reproducible standard method with good results has yet to be identified. Sperm quality assessment is essential for quantifying the impact of cryopreservation on spermatozoa. The purpose of our study was to provide additional information regarding semen cryopreservation and semen quality evaluation for the Russian sturgeon. Our study method is based on the use of two yolk-free extenders (with different cryoprotectants: DMSO and methanol) for freezing semen, using a simple freezing protocol. Parameters such as volume, concentration, motility, morphology and membrane integrity were evaluated. In conclusion, cryopreservation of Russian sturgeon spermatozoa using an extender containing methanol as cryoprotectant led to high egg fertilization percentages (72.67 ± 5.4%) even if the total motility values recorded for thawed semen were quite low (18-25%). Additionally, we identified two optimal stains for morphological studies and morphometry (Spermac stain kit and Trypan Blue Solution).

Indexed as

cryopreservationfertilization ratemorphologyRussian sturgeonsemen evaluation

Identifiers

PMID36009743
PMCPMC9405347
OpenAlexW4292693489

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.