Evidence map›Paper›PMID 36009638›Full record

ArticleAnimals : an open access journal from MDPI2022

Molecular Identification of

Alina Marandykina-Prakienė, Dalius Butkauskas, Naglis Gudiškis, Evelina Juozaitytė-Ngugu, Vytautas Januškevičius, Eglė Rudaitytė-Lukošienė, Petras Prakas

Open access · goldAbstract read
In one paragraph

Article in Animals : an open access journal from MDPI, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
3.7field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 23 citations in OpenAlex.

  1. Identification and characterization ofInternational journal for parasitology. Parasites and wildlife · 2026
    Article
  2. Pathogens (Basel, Switzerland) · 2026
    Article
  3. High Diversity ofAnimals : an open access journal from MDPI · 2026
    Article
  4. Molecular Evidence of the Role of the Red Fox (Animals : an open access journal from MDPI · 2026
    Article
  5. Microscopic and Molecular Identification ofAnimals : an open access journal from MDPI · 2026
    Article
  6. Molecular Screening ofAnimals : an open access journal from MDPI · 2025
    Article
  7. Article
  8. Detection ofFood and waterborne parasitology · 2025
    Article
  9. European Pine Marten (Veterinary sciences · 2025
    Article
  10. Molecular Evidence of Raccoon DogPathogens (Basel, Switzerland) · 2025
    Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Identification ofVeterinary sciences · 2024
    Article
  16. Article
  17. Veterinary sciences · 2023
    Article
  18. Article
  19. Molecular Identification of ProtozoanLife (Basel, Switzerland) · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Alina Marandykina-PrakienėNature Research Centre, Akademijos Str. 2, LT-08412 Vilnius, Lithuania.
Dalius ButkauskasNature Research Centre, Akademijos Str. 2, LT-08412 Vilnius, Lithuania.ORCID 0000-0003-2892-0493
Naglis GudiškisNature Research Centre, Akademijos Str. 2, LT-08412 Vilnius, Lithuania.
Evelina Juozaitytė-NguguNature Research Centre, Akademijos Str. 2, LT-08412 Vilnius, Lithuania.ORCID 0000-0002-7702-1817
Vytautas JanuškevičiusNature Research Centre, Akademijos Str. 2, LT-08412 Vilnius, Lithuania.
Eglė Rudaitytė-LukošienėNature Research Centre, Akademijos Str. 2, LT-08412 Vilnius, Lithuania.
Petras PrakasNature Research Centre, Akademijos Str. 2, LT-08412 Vilnius, Lithuania.
Nature Research Centre · LT

Funding

Lietuvos Mokslo Taryba Postdoc No.: 09.3.3-LMT-K-712-23-0064
6 · The paper itself

Abstract

Data on the distribution of different Sarcocystis species in various muscles of sheep are scarce. In the present study, 190 diaphragm, oesophagus, and heart muscle samples of 69 sheep raised in Lithuania were examined for the presence of Sarcocystis spp. Under a light microscope, two morphological types of microcysts corresponding to S. arieticanis and S. tenella were detected. Eight and 12 sarcocysts of S. arieticanis and S. tenella, respectively, were isolated and characterised by the sequencing of a portion of cox1. The sequence comparisons revealed the highest similarity between European and Asian isolates of S. arieticanis and S. tenella obtained from domestic sheep and other wild Caprinae hosts. Based on peptic digestion, nested PCR targeting cox1, and sequencing, a 100% infection prevalence of S. arieticanis and S. tenella was observed in the 69 studied animals. The occurrence of S. tenella was significantly higher in the diaphragm than in the oesophagus (χ2 = 13.14, p < 0.001), whereas differences in the prevalence of S. arieticanis in the studied muscle types were insignificant (χ2 = 1.28, p > 0.05). Further molecularly based epidemiological studies are needed to compare the prevalence of Sarcocystis species in various muscles of sheep raised in different geographic regions.

Indexed as

cox1domestic sheepepidemiologymolecular identificationSarcocystis arieticanisSarcocystis tenella

Identifiers

PMID36009638
PMCPMC9404443
OpenAlexW4290974121

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.