ArticleAntioxidants (Basel, Switzerland)2022
Functional Characterization of Arylalkylamine
Article in Antioxidants (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 13 citations in OpenAlex.
- Melatonin-Producing Microorganisms: A Rising Research Interest in Their Melatonin Biosynthesis and Effects on Crops.Journal of pineal research · 2025Review
- Effect of Exogenous Melatonin Supply on Potato Plants Grown In Vitro.Antioxidants (Basel, Switzerland) · 2025Article
- Research progress on melatonin, 5-HT, and orexin in sleep disorders of children with autism spectrum disorder.Biomolecules & biomedicine · 2025Review
- Evolutionary formation of melatonin and vitamin D in early life forms: insects take centre stage.Biological reviews of the Cambridge Philosophical Society · 2024Review
- Bioactive Peptides in Dairy Milk: Highlighting the Role of Melatonin.Biomolecules · 2024Review
- Mutagenesis selection and large-scale cultivation of non-greenFrontiers in nutrition · 2024Article
- Article
- Human Naa50 Shows SerotoninAntioxidants (Basel, Switzerland) · 2023Article
- Melatonin from Microorganisms, Algae, and Plants as Possible Alternatives to Synthetic Melatonin.Metabolites · 2023Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Arylalkylamine N-acetyltransferase (AANAT) is a pivotal enzyme in melatonin biosynthesis that catalyzes the conversion of serotonin to N-acetylserotonin. Homologs of animal AANAT genes are present in animals, but not in plants. An AANAT homolog was found in Chlamydomonas reinhardtii, but not other green algae. The characteristics of C. reinhardtii AANAT (CrAANAT) are unclear. Here, full-length CrAANAT was chemically synthesized and expressed in Escherichia coli. Recombinant CrAANAT exhibited AANAT activity with a Km of 247 μM and Vmax of 325 pmol/min/mg protein with serotonin as the substrate. CrAANAT was localized to the cytoplasm in tobacco leaf cells. Transgenic rice plants overexpressing CrAANAT (CrAANAT-OE) exhibited increased melatonin production. CrAANAT-OE plants showed a longer seed length and larger second leaf angle than wild-type plants, indicative of the involvement of brassinosteroids (BRs). As expected, BR biosynthesis- and signaling-related genes such as D2, DWARF4, DWARF11, and BZR1 were upregulated in CrAANAT-OE plants. Therefore, an increased endogenous melatonin level by ectopic overexpression of CrAANAT seems to be closely associated with BR biosynthesis, thereby influencing seed size.
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Registered trials
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