Evidence map›Paper›PMID 36009016›Full record

ArticleBiomolecules2022

High-Resolution Magic Angle Spinning NMR of KcsA in Liposomes: The Highly Mobile C-Terminus.

Gary S Howarth, Ann E McDermott

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomolecules, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Journal of biomolecular NMR · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Gary S HowarthDepartment of Chemistry, Columbia University, New York, NY 10031, USA.ORCID 0000-0002-3587-0546
Ann E McDermottDepartment of Chemistry, Columbia University, New York, NY 10031, USA.

Funding

TRD #3: An automated and streamlined pipeline for in-situ molecular microscopyP41GM103310 · NIGMS · SCRIPPS RESEARCH INSTITUTE, THE · PI KIEFT, JEFFREY S · 2012 to 2021
$14.7M
TR&D4: Integrated pipeline for data analysisP41GM118302 · NIGMS · NEW YORK STRUCTURAL BIOLOGY CENTER · PI PALMER, ARTHUR G · 2017 to 2021
$8.5M
RM1 Center on Macromolecular Dynamics by NMR Spectroscopy at the New York Structural Biology Center (CoMD/NMR)- Equipment Supplement RequestRM1GM145397 · NIGMS · NEW YORK STRUCTURAL BIOLOGY CENTER · PI ARTHUR G PALMER · 2022 to 2026
$8.3M
Structural and Functional Studies of Potassium Channels by Solid State NMRR01GM088724 · NIGMS · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI ANN E MCDERMOTT · 2009 to 2026
$5.6M
900 MHz NMR Spectrometer for Structural BiologyP41GM066354 · NIGMS · NEW YORK STRUCTURAL BIOLOGY CENTER · PI COWBURN, DAVID · 2002 to 2006
$4.7M
EXTRAMURAL RESEARCH FACILITIES CONSTRUCTIONC06RR015495 · NCRR · NEW YORK STRUCTURAL BIOLOGY CENTER · PI RIFKIND, RICHARD A · 2000 to 2000
$2.0M
RES FACIL CONSTRUCTION: HALLUCINOGENSC06RR017528 · NCRR · NEW YORK STRUCTURAL BIOLOGY CENTER · PI COWBURN, DAVID · 2002 to 2002
$2.0M
Upgrade of 800 MHz SpectrometerS10OD016432 · OD · NEW YORK STRUCTURAL BIOLOGY CENTER · PI PALMER, ARTHUR G · 2014 to 2014
$600k
NCRR NIH HHS C06 RR015495NCRR NIH HHS C06 RR017528NIGMS NIH HHS GM103310NIGMS NIH HHS P41 GM066354NIGMS NIH HHS P41 GM103310NIGMS NIH HHS P41 GM118302NIGMS NIH HHS R01 GM088724NIGMS NIH HHS RM1 GM145397NIH HHS C06RR017528-01-CEMNIH HHS CO6RR015495NIH HHS P41GM066354NIH HHS P41 GM-118302NIH HHS R01 GM088724NIH HHS S10 OD016432NIH HHS S10OD016432
6 · The paper itself

Abstract

The structure of the transmembrane domain of the pH-activated bacterial potassium channel KcsA has been extensively characterized, yet little information is available on the structure of its cytosolic, functionally critical N- and C-termini. This study presents high-resolution magic angle spinning (HR-MAS) and fractional deuteration as tools to study these poorly resolved regions for proteoliposome-embedded KcsA. Using

Indexed as

Bacterial ProteinsLiposomesHydrogen-Ion ConcentrationMagnetic Resonance SpectroscopyNuclear Magnetic Resonance, BiomolecularPotassium ChannelsBacterial ProteinsLiposomesPotassium Channelshigh-resolution nuclear magnetic resonance (HR-MAS NMR)KcsAlipid chemical shiftslipid hydrolysisliposomesmembrane protein structuresolid-state nuclear magnetic resonance (SS NMR)

Identifiers

PMID36009016
PMCPMC9405666

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.