Evidence map›Paper›PMID 36008739›Full record

ArticleMikrochimica acta2022

Rapid and simultaneous visual typing of high-risk HPV-16/18 with use of integrated lateral flow strip platform.

Qi Chen, Li Yao, Qian Wu, Jianguo Xu, Chao Yan, Chuanxiang Guo, Chao Zhang, Tao Xu, Panzhu Qin, Wei Chen

Abstract read
In one paragraph

Article in Mikrochimica acta, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
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  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Qi ChenEngineering Research Center of Bio-Process, MOE; School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China.
Li YaoEngineering Research Center of Bio-Process, MOE; School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China.
Qian WuEngineering Research Center of Bio-Process, MOE; School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China.
Jianguo XuEngineering Research Center of Bio-Process, MOE; School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China.
Chao YanEngineering Research Center of Bio-Process, MOE; School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China.
Chuanxiang GuoAnhui Deepblue Medical Technology Co. Ltd., Hefei, 230088, People's Republic of China.
Chao ZhangAnhui Deepblue Medical Technology Co. Ltd., Hefei, 230088, People's Republic of China.
Tao XuAnhui Medical University, Hefei, 230009, People's Republic of China.
Panzhu QinEngineering Research Center of Bio-Process, MOE; School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China. 345162615@qq.com.
Wei ChenEngineering Research Center of Bio-Process, MOE; School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China. chenweishnu@163.com.ORCID http://orcid.org/0000-0003-3763-1183

Funding

National Natural Science Foundation of China 32172295
6 · The paper itself

Abstract

A biosensor for rapid and simultaneous visual identification of high-risk human papillomavirus (HPV) genotypes 16 and 18 in clinical samples based on polymerase chain reaction (PCR) integrated lateral flow strip platform was developed. Using an one-step protocol to extract nucleic acid rapidly and the functionalized primer sets specific to HPV-16 and 18 were designed for the simultaneous amplification. In the presence of target HPV genotypes, the corresponding functionalized primer sets will participate in the PCR process and produce numerous duplex functionalized dsDNA amplicons. With the bridge effect of duplex functionalized dsDNA amplicons between gold nanoparticles-fluorescein isothiocyanate antibody conjugates (AuNP-FITC antibody conjugates) and other two antibodies on corresponding test line (T

Indexed as

Metal NanoparticlesPapillomavirus InfectionsDNAGoldHuman papillomavirus 16Human papillomavirus 18HumansDNAGoldHuman papillomavirusMolecular amplificationNucleic acid lateral flow stripRapid screeningTwo-cancer screeningVisualized sensing

Identifiers

PMID36008739
PMCPMC9409624

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.