ArticleNature communications2022
Tripterygium wilfordii cytochrome P450s catalyze the methyl shift and epoxidations in the biosynthesis of triptonide.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 29 papers.
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29 citing papers in PubMed, 52 citations in OpenAlex.
- Biosynthesis and heterologous production of the α-agarofuran scaffold of Celangulin V from Celastrus angulatus.Nature communications · 2026Article
- A Review of Plant-Derived Diterpenoid Biosynthesis: From Structural Scaffold Diversity and Lineage-Associated Distribution to Enzyme Mining and Discovery Strategies.Molecules (Basel, Switzerland) · 2026Review
- Loss and gain: directing the biosynthesis of metabolites in medicinal plants toward specific targets.Science China. Life sciences · 2026Article
- Transcription factor McORA activates McHDR to enhance aphid-repelling (E)-β-farnesene biosynthesis in Matricaria chamomilla L.Plant cell reports · 2026Article
- Discovery of Coracanols A and B, Two Biological Nitrification Inhibition Diterpenoids from Finger Millet.Organic letters · 2026Article
- Efficient biosynthesis of the plant-derived diterpenoid 14-hydroxy-dehydroabietadiene in Saccharomyces cerevisiae.Microbial cell factories · 2026Article
- Tripterygium glycosides: recent advances in mechanisms, therapeutic applications, and safety optimization.Frontiers in medicine · 2026Review
- Major bioactive metabolites derived fromFrontiers in pharmacology · 2026Review
- Gamma-selinene synthase catalyzes the first step of dihydroagarofuran sesquiterpene alkaloid biosynthesis in Tripterygium.Science China. Life sciences · 2025Article
- Cytochrome P450-Induced Backbone Rearrangements in Terpene Biosynthesis of Plants.Molecules (Basel, Switzerland) · 2025Review
- Advanced Pharmaceutical Nanotechnologies Applied for Chinese Herbal Medicines.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Heterologous Production of the Structurally Complex Diterpenoid Forskolin inBioengineering (Basel, Switzerland) · 2025Article
- Targeted metabolic engineering of key biosynthetic genes improves triptolide production in Tripterygium wilfordii hairy roots.Plant cell reports · 2025Article
- Advances in the Biosynthesis of Plant Terpenoids: Models, Mechanisms, and Applications.Plants (Basel, Switzerland) · 2025Review
- Genome-wide characterization and expression analysis of WRKY family genes in the biosynthesis of triptolide in Tripterygium wilfordii.BMC genomics · 2025Article
- Synthesis of AgInSDiscover oncology · 2025Article
- Combined metabolomic and transcriptomic analysis reveals the key genes for triterpenoid biosynthesis in Cyclocarya paliurus.BMC genomics · 2024Article
- Cutting-edge plant natural product pathway elucidation.Current opinion in biotechnology · 2024Review
- A pilot oral history of plant synthetic biology.Plant physiology · 2024Review
- Miltiradiene Production by Cytoplasmic Metabolic Engineering inMetabolites · 2023Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The diterpenoid triepoxides triptolide and triptonide from Tripterygium wilfordii (thunder god wine) exhibit unique bioactivities with potential uses in disease treatment and as a non-hormonal male contraceptives. Here, we show that cytochrome P450s (CYPs) from the CYP71BE subfamily catalyze an unprecedented 18(4→3) methyl shift required for biosynthesis of the abeo-abietane core structure present in diterpenoid triepoxides and in several other plant diterpenoids. In combination with two CYPs of the CYP82D subfamily, four CYPs from T. wilfordii are shown to constitute the minimal set of biosynthetic genes that enables triptonide biosynthesis using Nicotiana benthamiana and Saccharomyces cerevisiae as heterologous hosts. In addition, co-expression of a specific T. wilfordii cytochrome b
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