ArticleMicrobiology spectrum2022
Comparison of Different Label-Free Raman Spectroscopy Approaches for the Discrimination of Clinical MRSA and MSSA Isolates.
Article in Microbiology spectrum, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it, 21 citations in OpenAlex.
- Recent advances in surface enhanced Raman spectroscopy for bacterial pathogen identifications.Journal of advanced research · 2023Pooled it
- Vibrational spectroscopic characterisation of fluorescent-protein-tagged and wild-type bacteria in surface-associated microdroplets.Faraday discussions · 2026Article
- Early Sepsis Diagnosis as a Global Imperative: The Role of Raman Spectroscopy.Journal of clinical medicine · 2026Review
- Label-Free Differentiation of Antimicrobial Resistance Groups Using Raman Spectroscopy.Analytical chemistry · 2026Article
- Staphylococcus aureus's golden-yellow pigment staphyloxanthin: production enhancement, analytical characterization, and biological attributes.Microbial cell factories · 2026Review
- A Pilot Study on Single-Cell Raman Spectroscopy Combined with Machine Learning for Phenotypic Characterization ofMicroorganisms · 2025Article
- Nanoplasmonic SERS on fidget spinner for digital bacterial identification.Microsystems & nanoengineering · 2025Article
- Lighting the Path: Raman Spectroscopy's Journey Through the Microbial Maze.Molecules (Basel, Switzerland) · 2024Article
- Illuminating the Tiny World: A Navigation Guide for Proper Raman Studies on Microorganisms.Molecules (Basel, Switzerland) · 2024Review
- Rapid Identification of Pathogens Causing Bloodstream Infections by Raman Spectroscopy and Raman Tweezers.Microbiology spectrum · 2023Article
- Molecular Characteristics of Methicillin-Resistant and SusceptiblePathogens (Basel, Switzerland) · 2023Article
- Antibiotic Resistance Diagnosis in ESKAPE Pathogens-A Review on Proteomic Perspective.Diagnostics (Basel, Switzerland) · 2023Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) is classified as one of the priority pathogens that threaten human health. Resistance detection with conventional microbiological methods takes several days, forcing physicians to administer empirical antimicrobial treatment that is not always appropriate. A need exists for a rapid, accurate, and cost-effective method that allows targeted antimicrobial therapy in limited time. In this pilot study, we investigate the efficacy of three different label-free Raman spectroscopic approaches to differentiate methicillin-resistant and -susceptible clinical isolates of S. aureus (MSSA). Single-cell analysis using 532 nm excitation was shown to be the most suitable approach since it captures information on the overall biochemical composition of the bacteria, predicting 87.5% of the strains correctly. UV resonance Raman microspectroscopy provided a balanced accuracy of 62.5% and was not sensitive enough in discriminating MRSA from MSSA. Excitation of 785 nm directly on the petri dish provided a balanced accuracy of 87.5%. However, the difference between the strains was derived from the dominant staphyloxanthin bands in the MRSA, a cell component not associated with the presence of methicillin resistance. This is the first step toward the development of label-free Raman spectroscopy for the discrimination of MRSA and MSSA using single-cell analysis with 532 nm excitation.
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