Evidence map›Paper›PMID 36005449›Full record

ArticleMicrobiology spectrum2022

Engineered Fluorescent Strains of Cryptococcus neoformans: a Versatile Toolbox for Studies of Host-Pathogen Interactions and Fungal Biology, Including the Viable but Nonculturable State.

Raffael Júnio Araújo de Castro, Marco Túlio Aidar Mariano Rêgo, Fabiana S Brandão, Ana Laura Alfonso Pérez, Janice Lisboa De Marco, Marcio José Poças-Fonseca, Connie Nichols, J Andrew Alspaugh, Maria Sueli S Felipe, Alexandre Alanio and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiology spectrum, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.6field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 4 citations in OpenAlex.

  1. Is Cryptococcus neoformans a pleomorphic fungus?Current opinion in microbiology · 2024
    Review
  2. Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 3 countries.

Raffael Júnio Araújo de Castro *Laboratory of Applied Immunology, Campus Darcy Ribeiro, University of Brasíliagrid.7632.0, Asa Norte, Brasília, Federal District, Brazil.ORCID 0000-0002-5207-8892
Marco Túlio Aidar Mariano Rêgo *Laboratory of Applied Immunology, Campus Darcy Ribeiro, University of Brasíliagrid.7632.0, Asa Norte, Brasília, Federal District, Brazil.
Fabiana S BrandãoFaculty of Health Science, Campus Darcy Ribeiro, University of Brasíliagrid.7632.0, Asa Norte, Brasília, Federal District, Brazil.
Ana Laura Alfonso PérezDepartment of Cell Biology, Institute of Biological Sciences, Campus Darcy Ribeiro, University of Brasíliagrid.7632.0, Asa Norte, Brasilia, Federal District, Brazil.
Janice Lisboa De MarcoDepartment of Cell Biology, Institute of Biological Sciences, Campus Darcy Ribeiro, University of Brasíliagrid.7632.0, Asa Norte, Brasilia, Federal District, Brazil.
Marcio José Poças-FonsecaDepartment of Genetics and Morphology, Institute of Biological Sciences, Campus Darcy Ribeiro, University of Brasíliagrid.7632.0, Asa Norte, Brasília, Federal District, Brazil.ORCID 0000-0002-5207-8892
Connie NicholsDuke University School of Medicine, Department of Medicine, Durham, North Carolina, USA.
J Andrew AlspaughDuke University School of Medicine, Department of Medicine, Durham, North Carolina, USA.ORCID 0000-0003-3009-627X
Maria Sueli S FelipeCatholic University of Brasilia, Campus Asa Norte, Asa Norte, Brasília, Federal District, Brazil.
Alexandre AlanioCNRS, Unité de Mycologie Moléculaire, Centre National de Référence Mycoses et Antifongiques, Institut Pasteurgrid.428999.7, Paris, France.ORCID 0000-0001-9726-3082
Anamélia Lorenzetti BoccaLaboratory of Applied Immunology, Campus Darcy Ribeiro, University of Brasíliagrid.7632.0, Asa Norte, Brasília, Federal District, Brazil.ORCID 0000-0003-3323-5300
Larissa FernandesLaboratory of Applied Immunology, Campus Darcy Ribeiro, University of Brasíliagrid.7632.0, Asa Norte, Brasília, Federal District, Brazil.ORCID 0000-0002-2814-7501
Universidade de Brasília · BRCentre National de la Recherche Scientifique · FRDuke University · USUniversidade Católica de Brasília · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cryptococcus neoformans is an opportunistic fungal pathogen known for its remarkable ability to infect and subvert phagocytes. This ability provides survival and persistence within the host and relies on phenotypic plasticity. The viable but nonculturable (VBNC) phenotype was recently described in C. neoformans, whose study is promising in understanding the pathophysiology of cryptococcosis. The use of fluorescent strains is improving host interaction research, but it is still underexploited. Here, we fused histone H3 or the poly(A) binding protein (Pab) to enhanced green fluorescent protein (eGFP) or mCherry, obtaining a set of C. neoformans transformants with different colors, patterns of fluorescence, and selective markers (hygromycin B resistance [Hyg

Indexed as

CryptococcosisCryptococcus neoformansAnimalsBiologyHistonesHost-Pathogen InteractionsHumansHygromycin BMiceNeomycinHistonesHygromycin BNeomycinCryptococcus neoformansdormancyfluorescently tagged strainsGFPH99histone H3mCherrypoly(A) binding proteinstress granulesVBNC

Identifiers

PMID36005449
PMCPMC9603711
OpenAlexW4293101488

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.