Evidence map›Paper›PMID 36005038›Full record

ArticleBiosensors2022

A Gene Circuit Combining the Endogenous I-E Type CRISPR-Cas System and a Light Sensor to Produce Poly-β-Hydroxybutyric Acid Efficiently.

Xiaomeng Li, Wei Jiang, Qingsheng Qi, Quanfeng Liang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Xiaomeng LiState Key Laboratory of Microbial Technology, Shandong University, No. 72, Binhai Road, Qingdao 266237, China.
Wei JiangResearch Center of Basic Medicine, Central Hospital Affiliated to Shandong First Medical University, No. 105, Jiefang Road, Jinan 250013, China.
Qingsheng QiState Key Laboratory of Microbial Technology, Shandong University, No. 72, Binhai Road, Qingdao 266237, China.
Quanfeng LiangState Key Laboratory of Microbial Technology, Shandong University, No. 72, Binhai Road, Qingdao 266237, China.ORCID 0000-0002-4289-2508

Funding

National Key Research and Development Program of China 2021YFC2103600National Natural Science Foundation of China 31961133014, 31770095, 31971336
6 · The paper itself

Abstract

'Metabolic burden,' which arises when introducing exogenic synthesizing pathways into a host strain, remains a challenging issue in metabolic engineering. Redirecting metabolic flux from cell growth to product synthesis at an appropriate culture timepoint is ideal for resolving this issue. In this report, we introduce optogenetics-which is capable of precise temporal and spatial control-as a genetic switch, accompanied by the endogenous type I-E CRISPRi system in

Indexed as

CRISPR-Cas SystemsEscherichia coliGene Regulatory NetworksHydroxybutyratesPolyestersPolyhydroxybutyratesHydroxybutyratespoly-beta-hydroxybutyratePolyestersPolyhydroxybutyratesCcaS-CcaR systemmetabolic engineeringoptogeneticsPHBtype I-E CRISPR system

Identifiers

PMID36005038
PMCPMC9405541

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.