Evidence map›Paper›PMID 36005020›Full record

ArticleBiosensors2022

A Redox-Probe-Free Immunosensor Based on Electrocatalytic Prussian Blue Nanostructured Film One-Step-Prepared for Zika Virus Diagnosis.

Lorenna K B Santos, Priscila D Mendonça, LiLian K S Assis, Carlos R Prudêncio, Maria Izabel F Guedes, Ernesto T A Marques, Rosa Fireman Dutra

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
2.6field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 2 countries.

Lorenna K B SantosBiomedical Engineering Laboratory, Department of Biomedical Engineering, Federal University of Pernambuco, Avenida Professor Moraes Rego 1235, Recife 50670-90, Brazil.
Priscila D MendonçaBiomedical Engineering Laboratory, Department of Biomedical Engineering, Federal University of Pernambuco, Avenida Professor Moraes Rego 1235, Recife 50670-90, Brazil.
LiLian K S AssisBiomedical Engineering Laboratory, Department of Biomedical Engineering, Federal University of Pernambuco, Avenida Professor Moraes Rego 1235, Recife 50670-90, Brazil.ORCID 0000-0003-4547-0914
Carlos R PrudêncioImmunology Center, Institute Adolfo Lutz, São Paulo 01246-902, Brazil.ORCID 0000-0001-7701-0707
Maria Izabel F GuedesHuman Biochemistry Laboratory, University of Ceará, Fortaleza 60714-903, Brazil.
Ernesto T A MarquesDepartment of Infectious Diseases and Microbiology, University of Pittsburgh, Pittsburgh, PA 15261, USA.ORCID 0000-0003-3826-9358
Rosa Fireman DutraBiomedical Engineering Laboratory, Department of Biomedical Engineering, Federal University of Pernambuco, Avenida Professor Moraes Rego 1235, Recife 50670-90, Brazil.ORCID 0000-0001-8314-4218
Universidade Federal de Pernambuco · BRInstituto Adolfo Lutz · BRUniversidade Federal do Ceará · BRUniversity of Pittsburgh · US

Funding

Coordenação de Aperfeicoamento de Pessoal de Nível Superior Zika Virus grantNational Council for Scientific and Technological Development 44060520164
6 · The paper itself

Abstract

The Zika virus (ZIKV) is a great concern for global health due to its high transmission, including disseminating through blood, saliva, urine, semen and vertical transmission. In some cases, ZIKV has been associated with microcephaly, neurological disorders, and Guillain−Barré syndrome. There is no vaccine, and controlling the disease is a challenge, especially with the co-circulation of the Dengue virus, which causes a severe cross-reaction due to the similarity between the two arboviruses. Considering that electrochemical immunosensors are well-established, sensitive, and practical tools for diagnosis, in this study we developed a sensor platform with intrinsic redox activity that facilitates measurement readouts. Prussian blue (PB) has a great ability to form electrocatalytic surfaces, dispensing redox probe solutions in voltammetric measurements. Herein, PB was incorporated into a chitosan−carbon nanotube hybrid, forming a nanocomposite that was drop-casted on a screen-printed electrode (SPE). The immunosensor detected the envelope protein of ZIKV in a linear range of 0.25 to 1.75 µg/mL (n = 8, p < 0.01), with a 0.20 µg/mL limit of detection. The developed immunosensor represents a new method for electrochemical measurements without additional redox probe solutions, and it is feasible for application in point-of-care diagnosis.

Indexed as

Biosensing TechniquesZika VirusZika Virus InfectionFerrocyanidesHumansImmunoassayOxidation-Reductionferric ferrocyanideFerrocyanidesenvelope proteinimmunosensorpoint-of-care diagnosisPrussian blueZika virus

Identifiers

PMID36005020
PMCPMC9406047
OpenAlexW4290996790

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.