Fluorescence-Based Measurements of Membrane-Bound Angiotensin Converting Enzyme 2 Activity Using Xenopus Laevis Oocytes.

Open access · goldAbstract read

In one paragraph

Article in Biosensors, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact, top 82% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 2 countries.

Luise FastDepartment of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, 04103 Leipzig, Germany.ORCID 0000-0001-5681-292X

Richard ÅgrenDepartment of Neuroscience, Karolinska Institutet, 17177 Stockholm, Sweden.

Hugo ZebergDepartment of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, 04103 Leipzig, Germany.ORCID 0000-0001-7118-1249

Karolinska Institutet · SEMax Planck Institute for Evolutionary Anthropology · DE

Funding

Åhlén-stiftelsen 213066Fredrik och Ingrid Thurings Stiftelse 2020-00625 and 2021-00683Magnus Bergvalls stiftelse 2020-04055 and 2021-04489Stiftelsen Lars Hiertas Minne FO2020-0289 and FO2021-0400Swedish Research Council 2021-03050

6 · The paper itself

Abstract

Functional investigations of enzymes involving cellular expression systems are important for pharmacological studies. The precise control of expression is challenging in transiently transfected mammalian cell lines. Here, we explored the ability of Xenopus laevis oocytes to express a membrane-bound enzyme for functional characterization using standard 96-well plates and a fluorescence-based plate reader assay. We microinjected oocytes with cRNA encoding the angiotensin converting enzyme 2 (ACE2) and measured the enzymatic activity in single oocytes using a commercial fluorescence-based assay. The injected oocytes showed up to a 50-fold increase in fluorescence compared to uninjected oocytes. This fluorescence intensity was dose-dependent on the amount of

Indexed as

Angiotensin-Converting Enzyme 2OocytesAnimalsFluorescenceMammalsRNA, ComplementaryXenopus laevisAngiotensin-Converting Enzyme 2RNA, Complementaryangiotensin converting enzyme 2enzymatic assayfluorescence intensitymicroplate readerXenopus laevis oocytes

Identifiers

PMID36004997

DOI10.3390/bios12080601

PMCPMC9405939

OpenAlexW4290037457

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.