ArticleBiophysical journal2023
Different membrane order measurement techniques are not mutually consistent.
Article in Biophysical journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 31 citations in OpenAlex.
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- Increasing the Compositional Heterogeneity of Single-Chain Amphiphile Membranes Supported by Coacervate Cores Alters Stability and Properties of the Hybrid Protocells.Langmuir : the ACS journal of surfaces and colloids · 2026Article
- Hypoxia Modulates Membrane Mechanics in Pancreatic Cancer.The Journal of membrane biology · 2025Article
- Biophysical Consequences for Exposure of Model Cell Membranes to Perfluoroalkyl Substances.The journal of physical chemistry. B · 2025Article
- Loss of lipid asymmetry facilitates plasma membrane blebbing by decreasing membrane lipid packing.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Local mapping of the nanoscale viscoelastic properties of fluid membranes by AFM nanorheology.Nature communications · 2025Article
- HBimmCue: A Versatile Fluorescent Probe for Multi-Scale Imaging of Lipid Polarity and Membrane Order in Inner Mitochondrial Membrane.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Molecules in the Serotonin-Melatonin Synthesis Pathway Have Distinct Interactions with Lipid Membranes.The journal of physical chemistry. B · 2025Article
- Article
- Ratiometric fluorescence nanoscopy and lifetime imaging of novel Nile Red analogs for analysis of membrane packing in living cells.Scientific reports · 2024Article
- Differential Effects of Soy Isoflavones on the Biophysical Properties of Model Membranes.The journal of physical chemistry. B · 2024Article
- Quantifying Fluorescence Lifetime Responsiveness of Environment-Sensitive Probes for Membrane Fluidity Measurements.The journal of physical chemistry. B · 2024Article
- Serinc5 Restricts HIV Membrane Fusion by Altering Lipid Order and Heterogeneity in the Viral Membrane.ACS infectious diseases · 2023Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
"Membrane order" is a term commonly used to describe the elastic and mechanical properties of the lipid bilayer, though its exact meaning is somewhat context- and method dependent. These mechanical properties of the membrane control many cellular functions and are measured using various biophysical techniques. Here, we ask if the results obtained from various techniques are mutually consistent. Such consistency cannot be assumed a priori because these techniques probe different spatial locations and different spatial and temporal scales. We evaluate the change of membrane order induced by serotonin using nine different techniques in lipid bilayers of three different compositions. Serotonin is an important neurotransmitter present at 100s of mM concentrations in neurotransmitter vesicles, and therefore its interaction with the lipid bilayer is biologically relevant. Our measurement tools include fluorescence of lipophilic dyes (Nile Red, Laurdan, TMA-DPH, DPH), whose properties are a function of membrane order; atomic force spectroscopy, which provides a measure of the force required to indent the lipid bilayer;
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