ArticleCurrent research in insect science2021
Streamlined phage display library protocols for identification of insect gut binding peptides highlight peptide specificity.
Article in Current research in insect science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 13 citations in OpenAlex.
- Generation of an Insecticidal Human Domain Antibody from a Phage Library TargetingCurrent issues in molecular biology · 2026Article
- Gut-binding peptides as potential tools to reduce virus binding to honey bee gut surface proteins.Applied and environmental microbiology · 2025Article
- On the origin of non-specific binders isolated in the selection of phage display peptide libraries.Frontiers in microbiology · 2025Review
- Cry1Ba1-mediated toxicity of transgenicFrontiers in insect science · 2023Article
- Peptide mediated, enhanced toxicity of a bacterial pesticidal protein against southern green stink bug.Microbial biotechnology · 2022Article
- Engineering of Cry3Bb1 provides mechanistic insights toward countering western corn rootworm resistance.Current research in insect science · 2022Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phage display libraries have been used to isolate insect gut binding peptides for use as pathogen transmission blocking agents, and to provide artificial anchors for increased toxicity of bacteria-derived pesticidal proteins. Previously, phage clones displaying enriched peptides were sequenced by Sanger sequencing. Here we present a streamlined protocol for identification of insect gut binding peptides, using insect-appropriate feeding strategies, with next generation sequencing and tailored bioinformatics analyses. The bioinformatics pipeline is designed to eliminate poorly enriched and false positive peptides, and to identify peptides predicted to be stable and hydrophilic. In addition to developing streamlined protocols, we also sought to address whether candidate gut binding peptides can bind to insects from more than one order, which is an important consideration for safe, practical use of peptide-modified pesticidal proteins. To this end, we screened phage display libraries for peptides that bind to the gut epithelia of two pest insects, the Asian citrus psyllid,
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