Evidence map›Paper›PMID 36002988›Full record

ArticleCell transplantation

A One-Step, Monolayer Culture and Chemical-Based Approach to Generate Insulin-Producing Cells From Human Adipose-Derived Stem Cells to Mitigate Hyperglycemia in STZ-Induced Diabetic Rats.

Ruei-Yue Liang, Kai-Ling Zhang, Ming-Hsi Chuang, Feng-Huei Lin, Tzu-Chien Chen, Jhih-Ni Lin, Ya-Jyun Liang, Yi-An Li, Chun-Hung Chen, Peggy Leh Jiunn Wong and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell transplantation. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 5 institutions in 1 country.

Ruei-Yue LiangDepartment of Stem Cell Applied Technology, Gwo Xi Stem Cell Applied Technology, Hsinchu, Taiwan.ORCID 0000-0002-9914-931X
Kai-Ling ZhangDepartment of Stem Cell Applied Technology, Gwo Xi Stem Cell Applied Technology, Hsinchu, Taiwan.
Ming-Hsi ChuangDepartment of Technology Management, Chung Hua University, Hsinchu, Taiwan.
Feng-Huei LinDepartment of Biomedical Engineering, College of Engineering and College of Medicine, National Taiwan University, Taipei, Taiwan.
Tzu-Chien ChenDepartment of Biomedical Engineering, College of Engineering and College of Medicine, National Taiwan University, Taipei, Taiwan.
Jhih-Ni LinDepartment of Biomedical Engineering, College of Engineering and College of Medicine, National Taiwan University, Taipei, Taiwan.
Ya-Jyun LiangDepartment of Biomedical Engineering, College of Engineering and College of Medicine, National Taiwan University, Taipei, Taiwan.
Yi-An LiDepartment of Biomedical Engineering, College of Engineering and College of Medicine, National Taiwan University, Taipei, Taiwan.
Chun-Hung ChenDepartment of Stem Cell Applied Technology, Gwo Xi Stem Cell Applied Technology, Hsinchu, Taiwan.
Peggy Leh Jiunn WongDepartment of Stem Cell Applied Technology, Gwo Xi Stem Cell Applied Technology, Hsinchu, Taiwan.
Shinn-Zong LinBioinnovation Center, Tzu Chi Foundation, Hualien, Taiwan.ORCID 0000-0002-4601-9933
Po-Cheng LinDepartment of Stem Cell Applied Technology, Gwo Xi Stem Cell Applied Technology, Hsinchu, Taiwan.
Stem Cell Technology (Taiwan) · TWNational Taiwan University · TWChung Hua University · TWNational Health Research Institutes · TWTzu Chi University · TW

Funding

EPA EP-D-18-001
6 · The paper itself

Abstract

The global population of individuals afflicted with diabetes mellitus has been increasing year by year, and this disease poses a serious threat to human health as well as the economies worldwide. Pancreatic or islet transplantations provide one of the most effective and long-term therapies available to treat diabetes, but the scarcity and quality of pancreatic islets limit their use in treatments. Here, we report the development of a one-step, monolayer culture, and chemical-based protocol that efficiently mediates the differentiation of human adipose-derived stem cells (hADSCs) into insulin-producing cells (IPCs). Our data indicate that hADSCs in monolayer culture that are allowed to differentiate into IPCs are superior to those in suspension cultures with respect to insulin secretion capacity (213-fold increase), cell viability (93.5 ± 3.27% vs. 41.67 ± 13.17%), and response to glucose stimulation. Moreover, the expression of genes associated with pancreatic lineage specification, such as

Indexed as

Diabetes Mellitus, ExperimentalHyperglycemiaInsulin-Secreting CellsAnimalsCell DifferentiationHumansInsulinRatsStem CellsStreptozocinInsulinStreptozocinadipose-derived stem cellschemical-baseddiabetes mellitusinsulin-producing cellsmonolayer culture

Identifiers

PMID36002988
PMCPMC9421045
OpenAlexW4293101631

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.