ArticleNeural development2022
A single-cell transcriptomic atlas of complete insect nervous systems across multiple life stages.
Article in Neural development, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 50 citations in OpenAlex.
- Orb2 RNA-binding activity promotes neural stem cell development and brain growth in Drosophila larvae.Cell reports · 2026Article
- Temporal neuronal differentiation programs safeguard neuronal diversity.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- From candidate genes to omics: Unbiased approaches reshaping arthropod Evo-Devo.Genetics and molecular biology · 2026Article
- Functional Division of Insect Blood Cells by Single-Cell RNA-Sequencing and Cell-Type-Specific FISH Markers.Cells · 2025Article
- The Hox Gene,Molecular biology of the cell · 2025Article
- Altering cell size asymmetry inbioRxiv : the preprint server for biology · 2025Article
- From whole bodies to single cells: A guide to transcriptomic approaches for ecology and evolutionary biology.Molecular ecology · 2025Review
- The CPEB ortholog Orb2 regulates brain size through the TRIM-NHL RNA-binding protein, Brain tumor.bioRxiv : the preprint server for biology · 2025Article
- The Hox Gene,bioRxiv : the preprint server for biology · 2025Article
- Sensation of electric fields in the Drosophila melanogaster larva.Current biology : CB · 2025Article
- Molecular profiling of invertebrate glia.Glia · 2025Review
- Conserved transcription factors coordinate synaptic gene expression through repression.bioRxiv : the preprint server for biology · 2025Article
- Imp/IGF2BP and Syp/SYNCRIP temporal RNA interactomes uncover combinatorial networks of regulators ofScience advances · 2025Article
- Trithorax regulates long-term memory in Drosophila through epigenetic maintenance of mushroom body metabolic state and translation capacity.PLoS biology · 2025Article
- The astrocyte-enriched geneFly · 2024Article
- Spatiotemporal changes in Netrin/Dscam1 signaling dictate axonal projection direction ineLife · 2024Article
- scRNA-seq data from the larval Drosophila ventral cord provides a resource for studying motor systems function and development.Developmental cell · 2024Article
- Article
- Evolution of chemosensory tissues and cells across ecologically diverse Drosophilids.Nature communications · 2024Article
- Baf-mediated transcriptional regulation of teashirt is essential for the development of neural progenitor cell lineages.Experimental & molecular medicine · 2024Article
Corrections and comments
- Erratum issued
Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
Abstract
Molecular profiles of neurons influence neural development and function but bridging the gap between genes, circuits, and behavior has been very difficult. Here we used single cell RNAseq to generate a complete gene expression atlas of the Drosophila larval central nervous system composed of 131,077 single cells across three developmental stages (1 h, 24 h and 48 h after hatching). We identify 67 distinct cell clusters based on the patterns of gene expression. These include 31 functional mature larval neuron clusters, 1 ring gland cluster, 8 glial clusters, 6 neural precursor clusters, and 13 developing immature adult neuron clusters. Some clusters are present across all stages of larval development, while others are stage specific (such as developing adult neurons). We identify genes that are differentially expressed in each cluster, as well as genes that are differentially expressed at distinct stages of larval life. These differentially expressed genes provide promising candidates for regulating the function of specific neuronal and glial types in the larval nervous system, or the specification and differentiation of adult neurons. The cell transcriptome Atlas of the Drosophila larval nervous system is a valuable resource for developmental biology and systems neuroscience and provides a basis for elucidating how genes regulate neural development and function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.