Evidence map›Paper›PMID 36002768›Full record

ReviewNature protocols2022

Constructing a cost-efficient, high-throughput and high-quality single-molecule localization microscope for super-resolution imaging.

John S H Danial, Jeff Y L Lam, Yunzhao Wu, Matthew Woolley, Eleni Dimou, Matthew R Cheetham, Derya Emin, David Klenerman

Erratum issuedAbstract readReview
PubMed Publisher
In one paragraph

Review in Nature protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed.

  1. Article
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  5. From Biophysics to Biomedical Physics.ACS bio & med chem Au · 2025
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

John S H Danial *Yusuf Hamied Department of Chemistry, University of Cambridge, Cambridge, UK. js2494@cam.ac.uk.ORCID http://orcid.org/0000-0002-9882-8441
Jeff Y L Lam *Yusuf Hamied Department of Chemistry, University of Cambridge, Cambridge, UK.ORCID http://orcid.org/0000-0001-7408-2342
Yunzhao Wu *Yusuf Hamied Department of Chemistry, University of Cambridge, Cambridge, UK.
Matthew WoolleyYusuf Hamied Department of Chemistry, University of Cambridge, Cambridge, UK.
Eleni DimouYusuf Hamied Department of Chemistry, University of Cambridge, Cambridge, UK.ORCID http://orcid.org/0000-0003-4885-135X
Matthew R CheethamYusuf Hamied Department of Chemistry, University of Cambridge, Cambridge, UK.
Derya EminYusuf Hamied Department of Chemistry, University of Cambridge, Cambridge, UK.
David KlenermanYusuf Hamied Department of Chemistry, University of Cambridge, Cambridge, UK. dk10012@cam.ac.uk.ORCID http://orcid.org/0000-0001-7116-6954

Funding

Medical Research Council
6 · The paper itself

Abstract

Single-molecule localization microscopy (SMLM) leverages the power of modern optics to unleash ultra-precise structural nanoscopy of complex biological machines in their native environments as well as ultra-sensitive and high-throughput medical diagnostics with the sensitivity of a single molecule. To achieve this remarkable speed and resolution, SMLM setups are either built by research laboratories with strong expertise in optical engineering or commercially sold at a hefty price tag. The inaccessibility of SMLM to life scientists for technical or financial reasons is detrimental to the progress of biological and biomedical discoveries reliant on super-resolution imaging. In this work, we present the NanoPro, an economic, high-throughput, high-quality and easy-to-assemble SMLM for super-resolution imaging. We show that our instrument performs similarly to the most expensive, best-in-class commercial microscopes and rivals existing open-source microscopes at a lower price and construction complexity. To facilitate its wide adoption, we compiled a step-by-step protocol, accompanied by extensive illustrations, to aid inexperienced researchers in constructing the NanoPro as well as assessing its performance by imaging ground-truth samples as small as 20 nm. The detailed visual instructions make it possible for students with little expertise in microscopy engineering to construct, validate and use the NanoPro in <1 week, provided that all components are available.

Indexed as

MicroscopySingle Molecule ImagingHumans

Identifiers

PMID36002768

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.