ArticleMolecular biology reports2022
Identification of GmGPATs and their effect on glycerolipid biosynthesis through seed-specific expression in soybean.
Article in Molecular biology reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 13 citations in OpenAlex.
- Genome-wide association study and transcriptomic analysis reveal new genes for unsaturated fatty acid contents in soybean.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Characterization of the SoybeanPlants (Basel, Switzerland) · 2025Article
- Genome-Wide Identification, Characterization, Evolutionary Analysis, and Expression Pattern of the GPAT Gene Family in Barley and Functional Analysis ofInternational journal of molecular sciences · 2024Article
- The functional divergence of homologous GPAT9 genes contributes to the erucic acid content of Brassica napus seeds.BMC plant biology · 2024Article
- Genome-Wide Tissue-Specific Genes Identification for Novel Tissue-Specific Promoters Discovery in Soybean.Genes · 2023Article
- Bioengineering of Soybean Oil and Its Impact on Agronomic Traits.International journal of molecular sciences · 2023Review
- Genetic basis of the oil biosynthesis in ultra-high-oil maize grains with an oil content exceeding 20.Frontiers in plant science · 2023Article
- Characterization and functional analysis ofFrontiers in plant science · 2023Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
backgroundGenetic improvement of soybean oil content depends on in-depth study of the glycerolipid biosynthesis pathway. The first acylation reaction catalysed by glycerol-3-phosphate acyltransferase (GPAT) is the rate-limiting step of triacylglycerol biosynthesis. However, the genes encoding GPATs in soybean remain unknown.
methodsWe used a novel yeast genetic complementation system and seed-specific heterologous expression to identify GmGPAT activity and molecular function in glycerolipid biosynthesis.
resultsSixteen GmGPAT genes were cloned by reverse transcription-PCR for screening in yeast genetic complementation. The results showed that GmGPAT9-2 could restore the conditional lethal double knockout mutant strain ZAFU1, and GmGPAT1-1 exhibited low acyltransferase activity in serial dilution assays. In addition, the spatiotemporal expression pattern of GmGPAT9-2 exhibited tissue specificity in leaves, flowers and seeds at different developmental stages. Furthermore, both the proportion of arachidic acid and erucic acid were significantly elevated in Arabidopsis thaliana transgenic lines containing the seed-specific GmGPAT9-2 compared wild type, but the oil content was not affected.
conclusionTogether, our results provide reference data for future engineering of triacylglycerol biosynthesis and fatty acid composition improvement through GPATs in soybean.
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