ArticleCell reports2022
A small RNA-guided PRC2 complex eliminates DNA as an extreme form of transposon silencing.
Article in Cell reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 40 citations in OpenAlex.
- The mechanistic and evolutionary diversity of programmed DNA elimination.Nature reviews. Genetics · 2026Review
- Dual roles of two Tfdps in germline nuclear meiosis and somatic nuclear fragmentation during sexual reproduction in the unicellular organismMarine life science & technology · 2026Article
- Notes on the life cycle of a new marine ciliate,Marine life science & technology · 2026Article
- The tiny germline chromosomes of Paramecium aurelia have an exceptionally high recombination rate and are capped by a new class of Helitrons.BMC biology · 2026Article
- A developmental condensin I complex assists the Paramecium PiggyMac domesticated transposase during programmed DNA elimination.Nucleic acids research · 2026Article
- A H3K27me3 reader complex couples H3K27me3 accumulation to nascent transcription of transposable elements in Paramecium.Genome biology · 2026Article
- Effector gene silencing coordinated by histone methylation and small RNAs enhances host adaptation in a plant pathogen.Nucleic acids research · 2026Article
- A histone methyltransferase-independent function of PRC2 controls small RNA dynamics during programmed DNA elimination in Paramecium.Nucleic acids research · 2025Article
- Soma-derived 30-nt small RNAs are coupled with chromosome breakage and precisely target nontransposon DNA against elimination inScience advances · 2025Article
- EHMT2 Controls Neural Crest-Derived Craniofacial Development but is Dispensable in Limb Development.bioRxiv : the preprint server for biology · 2025Article
- ISWI1 complex proteins facilitate developmental genome editing inGenome research · 2025Article
- The PIWI-interacting protein Gtsf1 controls the selective degradation of small RNAs in Paramecium.Nucleic acids research · 2025Article
- GTSF1 is required for transposon silencing in the unicellular eukaryote Paramecium tetraurelia.Nucleic acids research · 2024Article
- Two paralogous PHD finger proteins participate in natural genome editing in Paramecium tetraurelia.Journal of cell science · 2024Article
- RebL1 is required for macronuclear structure stability and gametogenesis inMarine life science & technology · 2024Article
- Application of RNA interference and protein localization to investigate housekeeping and developmentally regulated genes in the emerging model protozoan Paramecium caudatum.Communications biology · 2024Article
- From germline genome to highly fragmented somatic genome: genome-wide DNA rearrangement during the sexual process in ciliated protists.Marine life science & technology · 2024Article
- A SUMO E3 ligase promotes long non-coding RNA transcription to regulate small RNA-directed DNA elimination.eLife · 2024Article
- Programmed chromosome fragmentation in ciliated protozoa: multiple means to chromosome ends.Microbiology and molecular biology reviews : MMBR · 2023Review
- Inter-generational nuclear crosstalk links the control of gene expression to programmed genome rearrangement during the Paramecium sexual cycle.Nucleic acids research · 2023Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In animal germlines, transposons are silenced at the transcriptional or post-transcriptional level to prevent deleterious expression. Ciliates employ a more direct approach by physically eliminating transposons from their soma, utilizing piRNAs to recognize transposons and imprecisely excise them. Ancient, mutated transposons often do not require piRNAs and are precisely eliminated. Here, we characterize the Polycomb Repressive Complex 2 (PRC2) in Paramecium and demonstrate its involvement in the removal of transposons and transposon-derived DNA. Our results reveal a striking difference between the elimination of new and ancient transposons at the chromatin level and show that the complex may be guided by Piwi-bound small RNAs (sRNAs). We propose that imprecise elimination in ciliates originates from an ancient transposon silencing mechanism, much like in plants and metazoans, through sRNAs, repressive methylation marks, and heterochromatin formation. However, it is taken a step further by eliminating DNA as an extreme form of transposon silencing.
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