ArticleMicrobial cell factories2022
Enhancement of ergothioneine production by discovering and regulating its metabolic pathway in Cordyceps militaris.
Article in Microbial cell factories, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
- Metabolic engineering ofSynthetic and systems biotechnology · 2026Article
- Edible Fungi and Skin Redox Homeostasis: Bioactive Diversity, Processing, Oral Exposure, and Evidence Gaps.Antioxidants (Basel, Switzerland) · 2026Review
- Ergothioneine: Biosynthesis, Molecular Mechanisms, Physiological Function, and Role in Disease.MedComm · 2026Review
- High-Level Production of Ergothioneine in Yarrowia lipolytica via Systematic Metabolic Engineering Strategies.Biotechnology and bioengineering · 2026Article
- Current Evidence of Ergogenic and Post-Exercise Recovery Effects of Dietary Supplementation withNutrients · 2026Review
- The Conservation Crisis ofJournal of fungi (Basel, Switzerland) · 2025Review
- Identification and functional analysis of the SET domain-containing gene family in Cordyceps militaris.Scientific reports · 2025Article
- A Review of Novel Antioxidant Ergothioneine: Biosynthesis Pathways, Production, Function and Food Applications.Foods (Basel, Switzerland) · 2025Review
- Biosynthesis of ergothioneine: current state, achievements, and perspectives.Applied microbiology and biotechnology · 2025Review
- Anserine, Balenine, and Ergothioneine: Impact of Histidine-Containing Compounds on Exercise Performance-A Narrative Review.Nutrients · 2025Review
- Ergothioneine biosynthesis: The present state and future prospect.Synthetic and systems biotechnology · 2025Review
- Article
- AdvancingBioengineering (Basel, Switzerland) · 2024Review
- Effects of Drying Process and High Hydrostatic Pressure on Extraction of Antioxidant Ergothioneine fromFoods (Basel, Switzerland) · 2024Article
- Structure of mycobacterial ergothioneine-biosynthesis C-S lyase EgtE.The Journal of biological chemistry · 2024Article
- Discovering a novel glycosyltransferase geneFrontiers in microbiology · 2024Article
- Focus and Insights into the Synthetic Biology-Mediated Chassis of Economically Important Fungi for the Production of High-Value Metabolites.Microorganisms · 2023Review
- Application progress of CRISPR/Cas9 genome-editing technology in edible fungi.Frontiers in microbiology · 2023Review
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundCordyceps militaris is a traditional medicinal fungus contains a variety of functional ingredients and has been developed as an important mushroom food recently. Ergothioneine, one of the antioxidative compounds in C. militaris, is benefits on aging-related diseases and therefore became a novel functional food nutritive fortifier. Currently, the main diet source of ergothioneine is mushroom food. However, the mushroom farming faces the problems such as rather low ingredient yield and spontaneous degeneration associated fruiting body that restricts large scale production of ergothioneine.
resultsIn this study, we excavated the ergothioneine synthetases in mushroom and modified the genes in C. militaris to construct a new ergothioneine synthesis pathway. By further introducing this pathway into C. militaris genome, we succeeded to increase the ingredients' production of engineering strain, the highest amount of ergothioneine and cordycepin were up to 2.5 g/kg dry weight and 2 g/L, respectively. Additionally, the expression of ergothioneine synthetase genes in the shape-mutated degenerative C. militaris could recover the ability of degenerative strain to produce high amount of ingredients, suggesting the metabolic regulation of ergothioneine might release the symptom of mushroom degeneration.
conclusionThis study reveals a new pathway to fulfill the market needs of functional mushroom food and food fortifier ergothioneine. It implied the mycelium of C. militaris could be engineered as a novel medicinal mushroom food which could produce higher amount of valuable ingredients.
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