Evidence map›Paper›PMID 35999256›Full record

ArticleScientific reports2022

Phylogenetic analysis of Arthrospira strains from Ordos based on 16S rRNA.

Jingnan Zhang, Huiting Xue, Sirui Yan, Haibo Shi, Ling Du, Junfeng Zhang, Da Huo, Ruiping Hu, Hong Yuan, Chunli Ma

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Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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1 · What the graph read from it

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3 · Its place in the literature

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1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Jingnan Zhang *College of Basic Medicine, Inner Mongolia Medical University, Hohhot, 010110, China.
Huiting Xue *College of Basic Medicine, Inner Mongolia Medical University, Hohhot, 010110, China.
Sirui YanInner Mongolia Third Hospital, Inner Mongolia Medical University, Hohhot, 010110, China.
Haibo ShiCollege of Life Science and Technology, Inner Mongolia Normal University, Hohhot, 010022, China.
Ling DuCollege of Life Science and Technology, Inner Mongolia Normal University, Hohhot, 010022, China.
Junfeng ZhangCollege of Basic Medicine, Inner Mongolia Medical University, Hohhot, 010110, China.
Da HuoCollege of Basic Medicine, Inner Mongolia Medical University, Hohhot, 010110, China.
Ruiping HuCollege of Basic Medicine, Inner Mongolia Medical University, Hohhot, 010110, China. 783674348@qq.com.
Hong YuanCollege of Basic Medicine, Inner Mongolia Medical University, Hohhot, 010110, China. 44819680@qq.com.
Chunli MaCollege of Basic Medicine, Inner Mongolia Medical University, Hohhot, 010110, China. ma200614127@126.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

To study the growth rate of different Arthrospira strains, three species of Arthrospira from Ordos alkaline lake, labeled as sp.DD, sp.ER, sp.FB, one species of Arthrospira from Hasu Sea in Hohhot, labeled as sp.HS, another purified strain labeled as sp.QD donated by the Ocean University of China had been collected. The first four need to be further isolated and purified in culture. The growth curves of all strains were plotted. Subsequently, 16S rRNA sequences were amplified and sequenced in an attempt to study taxonomic relationships. The results showed that the growth rate was increased in the first 9 days, and sp.DD had the highest growth rate. Analysis of the sequencing results revealed that sp.HS had 99.79% homology with Arthrospira platensis strain Sp-2, sp.DD had 99.69% homology with Arthrospira platensis FACHB834, sp.QD had 99.54% homology with Arthrospira platensis F3S, sp.ER had 99.79% homology with Arthrospira erdosensis 'Inner Mongolia', sp.FB had 99.74% homology with Arthrospira erdosensis 'Inner Mongolia'. Phylogenetic analysis indicated that sp.HS was closely related to Arthrospira platensis strain Sp-2; sp.DD and sp.QD had a close genetic relationship; sp.ER and sp.FB had a close genetic relationship. In conclusion, these findings provide a theoretical basis for the further development and reproduction of dominant algae species in Inner Mongolia through biological analysis of Arthrospira.

Indexed as

SpirulinaDNA, BacterialPhylogenyRNA, Ribosomal, 16SSequence Analysis, DNADNA, BacterialRNA, Ribosomal, 16S

Identifiers

PMID35999256
PMCPMC9399139

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