ArticleCurrent protocols2022
A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae.
Article in Current protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 22 citations in OpenAlex.
- Pathogenicity and virulence of the blast fungusVirulence · 2026Review
- Stress-induced nucleolar rejuvenation via chaperone-mediated segregation in a filamentous fungus.The Journal of cell biology · 2026Article
- Multi-proteomic profiling indicates potential regulatory signatures underlying rice resistance to Magnaporthe oryzae.The Plant journal : for cell and molecular biology · 2026Article
- A Rapid Genomic DNA Extraction Method for Direct Quantitative Polymerase Chain Reaction-Based On-Site Detection of Soil-Borne Fungal Disease.The plant pathology journal · 2026Article
- A practical guide to two-stage sporulation of Pyricularia oryzae: introducing a filter paper method and comparison with existing methods using strains from diverse grass hosts.Plant methods · 2025Article
- Design and Validation of Specific qPCR Primers for Soil-Borne and Apple Tree-Associated Phytopathogenic Fungi.The plant pathology journal · 2025Article
- Decapeptide Inducer Promotes the Conidiation of PhytopathogenicInternational journal of molecular sciences · 2025Article
- The phase-separating Magnaporthe oryzae MoSpa2 complex organizes actin nucleation centers for plant infection.The Plant cell · 2025Article
- The link between changing in host carbon allocation and resistance toPlant signaling & behavior · 2024Review
- The cell-end protein Tea4 spatially regulates hyphal branch initiation and appressorium remodeling in the blast fungusMolecular biology of the cell · 2024Article
- MoCbp7, a Novel Calcineurin B Subunit-Binding Protein, Is Involved in the Calcium Signaling Pathway and Regulates Fungal Development, Virulence, and ER Homeostasis inInternational journal of molecular sciences · 2023Article
- A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae.Current protocols · 2022Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The blast fungus, Magnaporthe oryzae, is a devastating plant pathogen that threatens global food security. The social and economic importance of blast disease has contributed to this filamentous fungus becoming a model organism for the study of host-pathogen interactions. Availability of the complete genome sequences of many strains of the pathogen, as well as rice and wheat cultivars, coupled with the tractability of M. oryzae to classical and molecular genetic manipulation have contributed to its widespread study. Although M. oryzae has been extensively investigated for the past two decades, procedures for storing, maintaining, and manipulating the blast fungus in the laboratory had not been compiled and updated. As a consequence, there is considerable disparity in how the fungus is stored and manipulated between studies. In this article, we present a collection of protocols providing clear explanations of how to preserve filter stocks of M. oryzae; how to grow the fungus in both liquid and solid media; how to extract genomic DNA from fungal mycelium; how to induce appressorium formation on coverslips for visualization and tissue collection; and how to perform two distinct types of plant infection assay for virulence assessment. By sharing our most used laboratory procedures, we aim to address some of the knowledge gaps in current M. oryzae protocols and contribute to uniformity and robustness in studies by the Magnaporthe research community. © 2022 The Authors. Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Storage of M. oryzae strains Basic Protocol 2: Revival and regular maintenance of M. oryzae cultures in solid medium Alternate Protocol 1: Regular maintenance of M. oryzae cultures in liquid medium Basic Protocol 3: Genomic DNA extraction from M. oryzae mycelium Alternate Protocol 2: Quick DNA extraction from M. oryzae mycelium Basic Protocol 4: M. oryzae induction of appressorium development on glass coverslips for microscopy Alternate Protocol 3: M. oryzae induction of appressorium development on glass coverslips for tissue collection Basic Protocol 5: M. oryzae rice infection assay through spray inoculation Alternate Protocol 4: M. oryzae leaf-drop plant infection assay.
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