ArticleBiotechnology for biofuels and bioproducts2022
Metabolic engineering of Escherichia coli for direct production of vitamin C from D-glucose.
Article in Biotechnology for biofuels and bioproducts, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 14 citations in OpenAlex.
- Efficient Production of 2-Keto-l-Gulonic Acid via One-Step Fermentation UsingMicroorganisms · 2026Article
- Enhancement of Growth, Antioxidant Activity, and Immunity in Nile Tilapia (Antioxidants (Basel, Switzerland) · 2025Article
- L-gulono-γ-lactone Oxidase, the Key Enzyme for L-Ascorbic Acid Biosynthesis.Current issues in molecular biology · 2024Review
- Recombinant C-Terminal Catalytic Domain of Rat L-Gulono Lactone Oxidase Produced in Bacterial Cells Is Enzymatically Active.Current issues in molecular biology · 2024Article
- The ascorbate biosynthesis pathway in plants is known, but there is a way to go with understanding control and functions.Journal of experimental botany · 2024Review
- Biosynthesis of melatonin from L-tryptophan by an engineered microbial cell factory.Biotechnology for biofuels and bioproducts · 2024Article
- Vitamin C: From nutrition to oxygen sensing and epigenetics.Redox biology · 2023Article
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundProduction of vitamin C has been traditionally based on the Reichstein process and the two-step process. However, the two processes share a common disadvantage: vitamin C cannot be directly synthesized from D-glucose. Therefore, significant effort has been made to develop a one-step vitamin C fermentation process. While, 2-KLG, not vitamin C, is synthesized from nearly all current one-step fermentation processes. Vitamin C is naturally synthesized from glucose in Arabidopsis thaliana via a ten-step reaction pathway that is encoded by ten genes. The main objective of this study was to directly produce vitamin C from D-glucose in Escherichia coli by expression of the genes from the A. thaliana vitamin C biosynthetic pathway.
resultsTherefore, the ten genes of whole vitamin C synthesis pathway of A. thaliana were chemically synthesized, and an engineered strain harboring these genes was constructed in this study. The direct production of vitamin C from D-glucose based on one-step fermentation was achieved using this engineered strain and at least 1.53 mg/L vitamin C was produced in shaking flasks.
conclusionsThe study demonstrates the feasibility of one-step fermentation for the production of vitamin C from D-glucose. Importantly, the one-step process has significant advantages compared with the currently used fermentation process: it can save multiple physical and chemical steps needed to convert D-glucose to D-sorbitol; it also does not involve the associated down-streaming steps required to convert 2-KLG into vitamin C.
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