ArticleCommunications biology2022
Label-free chemical imaging of cytochrome P450 activity by Raman microscopy.
Article in Communications biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
- Longitudinal live-cell chemical imaging using coherent Raman scattering microscopy.The Analyst · 2026Review
- Label-Free Hyperspectral Raman Imaging and Analysis of a 3D Spheroid Model for Liver Fibrosis Study.Chemical & biomedical imaging · 2026Article
- Broadband electronic resonance coherent anti-Stokes/Stokes Raman scattering microscopy.Science advances · 2026Article
- Advanced Imaging for Live-Cell Spatiotemporal Monitoring: Technologies and Applications.Research (Washington, D.C.) · 2026Review
- Single-cell Raman imaging reveals fructose impairs brown adipocyte differentiation.Biosensors & bioelectronics · 2025Article
- Integrating spatial and chemical information enhances differentiation of non-alcoholic steatohepatitis states in Raman imaging.Scientific reports · 2025Article
- Lipid droplets as cell fate determinants in skeletal muscle.Trends in endocrinology and metabolism: TEM · 2025Review
- Electrode- and Label-Free Assessment of Electrophysiological Firing Rates through Cytochrome C Monitoring via Raman Spectroscopy.ACS sensors · 2025Article
- Raman microscopy of cryofixed biological specimens for high-resolution and high-sensitivity chemical imaging.Science advances · 2024Article
- Label-Free Raman Spectroscopy for Assessing Purity and Maturation of hiPSC-Derived Cardiac Tissue.Analytical chemistry · 2024Article
- Noise learning of instruments for high-contrast, high-resolution and fast hyperspectral microscopy and nanoscopy.Nature communications · 2024Article
- P-ring: The conserved nature of phosphorus enriched cells in seedling roots of distantly related species.Plant signaling & behavior · 2023Article
- Recent advancements in single-cell metabolic analysis for pharmacological research.Journal of pharmaceutical analysis · 2023Review
- Strategies and Progress of Raman Technologies for Cellular Uptake Analysis of the Drug Delivery Systems.International journal of nanomedicine · 2023Review
- Raman Spectroscopy for Chemical Biology Research.Journal of the American Chemical Society · 2022Review
Corrections and comments
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Authors and funding
6 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although investigating drug modulation of cytochrome P450 (CYP) activity under physiological conditions is crucial in drug development to avoid severe adverse drug reactions, the current evaluation approaches that rely on the destructive and end-point analysis can be misleading due to invasive treatments and cellular heterogeneity. Here, we propose a non-destructive and high-content method for visualizing and quantifying intracellular CYP activity under drug administration by Raman microscopy. The redox-state and spin-state sensitive Raman measurement indicated that the induced CYPs in living hepatocytes were in oxidized and low-spin state, which is related to monooxygenase function of CYP. Moreover, glycogen depletion associated with CYP induction was simultaneously observed, indicating a relevant effect on glucose metabolism. By deciphering the overall changes in the biochemical fingerprints of hepatocytes, Raman microscopy offers a non-destructive and quantitative chemical imaging method to evaluate CYP activity at the single-cell level with the potential to facilitate future drug development schemes.
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Registered trials
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