Evidence map›Paper›PMID 35994655›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2022

In vitro reconstitution of calcium-dependent recruitment of the human ESCRT machinery in lysosomal membrane repair.

Sankalp Shukla, Kevin P Larsen, Chenxi Ou, Kevin Rose, James H Hurley

Open access · hybridAbstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.

0numbers the graph read from it
0cells of the map it votes in
47citing papers in PubMed
5.4field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

47 citing papers in PubMed, 52 citations in OpenAlex.

  1. Article
  2. Sorcin couples Annexin A11 recruitment to ESCRT-III assembly for plasma membrane repair.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  3. Article
  4. Article
  5. Review
  6. bioRxiv : the preprint server for biology · 2026
    Article
  7. Review
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. ESCRT-III function in membrane fission and repair.Nature reviews. Molecular cell biology · 2026
    Review
  14. Review
  15. Review
  16. Article
  17. Review
  18. Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Sankalp ShuklaDepartment of Molecular and Cell Biology, University of California, Berkeley, CA 94720.ORCID 0000-0002-1038-8681
Kevin P LarsenDepartment of Molecular and Cell Biology, University of California, Berkeley, CA 94720.ORCID 0000-0002-3464-2023
Chenxi OuDepartment of Molecular and Cell Biology, University of California, Berkeley, CA 94720.
Kevin RoseDepartment of Molecular and Cell Biology, University of California, Berkeley, CA 94720.ORCID 0000-0002-9373-4147
James H HurleyDepartment of Molecular and Cell Biology, University of California, Berkeley, CA 94720.ORCID 0000-0001-5054-5445
QB3 · US

Funding

MOLECULAR BIOPHYSICS TRAINING GRANTT32GM008295 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI HURLEY, JAMES H, WEMMER, DAVID E · 1989 to 2022
$8.1M
Biochemical, Biophysical, and Structural Mechanisms of HIV-1 Budding and ReleaseR37AI112442 · NIAID · UNIVERSITY OF CALIFORNIA BERKELEY · PI James H Hurley · 2019 to 2026
$4.3M
Determining the structural basis for ESCRT-mediated membrane scission in HIV-1 virion buddingF32AI155226 · NIAID · UNIVERSITY OF CALIFORNIA BERKELEY · PI LARSEN, KEVIN · 2020 to 2022
$194k
NIAID NIH HHS F32 AI155226NIAID NIH HHS R37 AI112442NIGMS NIH HHS T32 GM008295
6 · The paper itself

Abstract

The endosomal sorting complex required for transport (ESCRT) machinery is centrally involved in the repair of damage to both the plasma and lysosome membranes. ESCRT recruitment to sites of damage occurs on a fast time scale, and Ca

Indexed as

CalciumEndosomal Sorting Complexes Required for TransportIntracellular MembranesLysosomesApoptosis Regulatory ProteinsATPases Associated with Diverse Cellular ActivitiesBiological TransportCalcium-Binding ProteinsCell Cycle ProteinsHumansIn Vitro TechniquesApoptosis Regulatory ProteinsATPases Associated with Diverse Cellular ActivitiesCalciumCalcium-Binding ProteinsCell Cycle ProteinsCHMP2A protein, humanCHMP3 protein, humanCHMP4B protein, humanEndosomal Sorting Complexes Required for TransportPDCD6IP protein, humanPDCD6 protein, humanVPS4B protein, humanin vitro reconstitutionmembrane biologymembrane remodelingmembrane repairneurodegeneration

Identifiers

PMID35994655
PMCPMC9436306
OpenAlexW4292701969

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.