ArticleLangmuir : the ACS journal of surfaces and colloids2022
Bending Rigidity, Capacitance, and Shear Viscosity of Giant Vesicle Membranes Prepared by Spontaneous Swelling, Electroformation, Gel-Assisted, and Phase Transfer Methods: A Comparative Study.
Article in Langmuir : the ACS journal of surfaces and colloids, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 35 citations in OpenAlex.
- Electric-field manipulation of giant vesicles: new frontiers in membrane electromechanics.European biophysics journal : EBJ · 2026Review
- Morphogenic colloids.Nature communications · 2026Article
- Quantification of cholesterol incorporation in giant unilamellar vesicles produced by a modified cDICE method.Soft matter · 2026Article
- Polymerization from Lipid Membranes.Biomacromolecules · 2026Article
- Thermally Driven Dynamic Behaviors in Polymeric Vesicles.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Synthesis Method and High Salt Concentration Can Affect Electrodeformation of GUVs under Strong Pulsed DC Fields.ACS omega · 2025Article
- A New Means to Generate Liposomes by Rehydrating Engineered Lipid Nanoconstructs.Micromachines · 2025Article
- Effect of leaflet asymmetry on the stretching elasticity of lipid bilayers with phosphatidic acid.Biophysical journal · 2024Article
- Efficient calculation of orientation-dependent lipid dynamics from membrane simulations.bioRxiv : the preprint server for biology · 2024Article
- Membranes on the move: The functional role of the extracellular vesicle membrane for contact-dependent cellular signalling.Journal of extracellular vesicles · 2024Review
- Article
- Secondary Ion Mass Spectrometry of Single Giant Unilamellar Vesicles Reveals Compositional Variability.Journal of the American Chemical Society · 2023Article
- Article
- Photomanipulation of Minimal Synthetic Cells: Area Increase, Softening, and Interleaflet Coupling of Membrane Models Doped with Azobenzene-Lipid Photoswitches.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Toxic Effects of Penetrating Cations.Membranes · 2023Review
- Quantification of Giant Unilamellar Vesicle Fusion Products by High-Throughput Image Analysis.International journal of molecular sciences · 2023Article
- A Practical Guide to Preparation and Applications of Giant Unilamellar Vesicles Formed via Centrifugation of Water-in-Oil Emulsion Droplets.Membranes · 2023Review
- Assessing membrane material properties from the response of giant unilamellar vesicles to electric fields.Advances in physics: X · 2023Article
- Commentary: Rapid and facile preparation of giant vesicles by the droplet transfer method for artificial cell construction.Frontiers in bioengineering and biotechnology · 2022Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Closed lipid bilayers in the form of giant unilamellar vesicles (GUVs) are commonly used membrane models. Various methods have been developed to prepare GUVs, however it is unknown if all approaches yield membranes with the same elastic, electric, and rheological properties. Here, we combine flickering spectroscopy and electrodefomation of GUVs to measure, at identical conditions, membrane capacitance, bending rigidity and shear surface viscosity of palmitoyloleoylphosphatidylcholine (POPC) membranes formed by several commonly used preparation methods: thin film hydration (spontaneous swelling), electroformation, gel-assisted swelling using poly(vinyl alcohol) (PVA) or agarose, and phase-transfer. We find relatively similar bending rigidity value across all the methods except for the agarose hydration method. In addition, the capacitance values are similar except for vesicles prepared via PVA gel hydration. Intriguingly, membranes prepared by the gel-assisted and phase-transfer methods exhibit much higher shear viscosity compared to electroformation and spontaneous swelling, likely due to remnants of polymers (PVA and agarose) and oils (hexadecane and mineral) in the lipid bilayer structure.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.