Evidence map›Paper›PMID 35993403›Full record

ArticleThe Analyst2022

Portable electroanalytical nucleic acid amplification tests using printed circuit boards and open-source electronics.

Anna Toldrà, Alar Ainla, Shirin Khaliliazar, Roman Landin, Georgios Chondrogiannis, Martin Hanze, Pedro Réu, Mahiar M Hamedi

Open access · hybridAbstract read
In one paragraph

Article in The Analyst, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.7field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Anna ToldràSchool of Engineering Sciences in Chemistry, Biotechnology, and Health, KTH Royal Institute of Technology, Stockholm 10044, Sweden. mahiar@kth.se.ORCID http://orcid.org/0000-0001-6371-6055
Alar AinlaInternational Iberian Nanotechnology Laboratory, 4715-330 Braga, Portugal.
Shirin KhaliliazarSchool of Engineering Sciences in Chemistry, Biotechnology, and Health, KTH Royal Institute of Technology, Stockholm 10044, Sweden. mahiar@kth.se.
Roman LandinSchool of Engineering Sciences in Chemistry, Biotechnology, and Health, KTH Royal Institute of Technology, Stockholm 10044, Sweden. mahiar@kth.se.
Georgios ChondrogiannisSchool of Engineering Sciences in Chemistry, Biotechnology, and Health, KTH Royal Institute of Technology, Stockholm 10044, Sweden. mahiar@kth.se.
Martin HanzeSchool of Engineering Sciences in Chemistry, Biotechnology, and Health, KTH Royal Institute of Technology, Stockholm 10044, Sweden. mahiar@kth.se.
Pedro RéuSchool of Engineering Sciences in Chemistry, Biotechnology, and Health, KTH Royal Institute of Technology, Stockholm 10044, Sweden. mahiar@kth.se.ORCID http://orcid.org/0000-0001-7454-7189
Mahiar M HamediSchool of Engineering Sciences in Chemistry, Biotechnology, and Health, KTH Royal Institute of Technology, Stockholm 10044, Sweden. mahiar@kth.se.ORCID http://orcid.org/0000-0001-9088-1064
KTH Royal Institute of Technology · SEInternational Iberian Nanotechnology Laboratory · PT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The realization of electrochemical nucleic acid amplification tests (NAATs) at the point of care (POC) is highly desirable, but it remains a challenge given their high cost and lack of true portability/miniaturization. Here we show that mass-produced, industrial standardized, printed circuit boards (PCBs) can be repurposed to act as near-zero cost electrodes for self-assembled monolayer-based DNA biosensing, and further integration with a custom-designed and low-cost portable potentiostat. To show the analytical capability of this system, we developed a NAAT using isothermal recombinase polymerase amplification, bypassing the need of thermal cyclers, followed by an electrochemical readout relying on a sandwich hybridization assay. We used our sensor and device for analytical detection of the toxic microalgae

Indexed as

Biosensing TechniquesNucleic Acid Amplification TechniquesDNAElectronicsRecombinasesDNARecombinases

Identifiers

PMID35993403
PMCPMC9511072
OpenAlexW4291452745

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.