ReviewInterface focus2022
Current strategies with implementation of three-dimensional cell culture: the challenge of quantification.
Review in Interface focus, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed.
- Hyperpolarised magnetic resonance spectroscopy with lab-on-a-chip disease models.Disease models & mechanisms · 2026Review
- Microfluidic Control of Dorsal-Ventral Patterning Within a Single Forebrain Organoid.bioRxiv : the preprint server for biology · 2026Article
- Electrospun 3D Nanofiber Scaffolds with Adhesion-Enhanced Interfaces for Cell Culture.ACS omega · 2026Article
- In Vitro 3D Model of Human Endometrial Stromal and Trophoblast Cells: Techniques for an Optimized Formation and Cryopreservation of Spheroids.Methods and protocols · 2026Article
- Reference Gene Validation for Quantitative PCR Analysis in 2D and 3D AML12 Hepatocyte Models.Biomedicines · 2026Article
- Unveiling Particulate Matter-Lung Interactions from Static Models to Dynamic Lung-on-Chips.Research (Washington, D.C.) · 2026Review
- Release, Transfer, Fold: Using a Silicone Adhesive for On-Demand 3D Tissue Engineering.ACS biomaterials science & engineering · 2025Article
- Towards a quality control framework for cerebral cortical organoids.Scientific reports · 2025Article
- Ex Vivo Preconditioning as a Useful Tool for Modification of the Extracellular Matrix of Multipotent Mesenchymal Stromal Cells.International journal of molecular sciences · 2025Review
- Proposal for a non-adhesive single-cell culture technology for primary hepatocytes.Cytotechnology · 2025Article
- Understanding the cellular dynamics, engineering perspectives and translation prospects in bioprinting epithelial tissues.Bioactive materials · 2025Review
- Conductive Biocomposite Made by Two-Photon Polymerization of Hydrogels Based on BSA and Carbon Nanotubes with Eosin-Y.Gels (Basel, Switzerland) · 2024Article
- Article
- Agent-based model predicts that layered structure and 3D movement work synergistically to reduce bacterial load in 3D in vitro models of tuberculosis granuloma.PLoS computational biology · 2024Article
- Review
- Integrated Experimental and Mathematical Exploration of Modular Tissue Cultures for Developmental Engineering.International journal of molecular sciences · 2024Article
- A comprehensive overview of advanced dynamicTissue barriers · 2024Review
- Plant Cellulose as a Substrate for 3D Neural Stem Cell Culture.Bioengineering (Basel, Switzerland) · 2023Article
- Correlative Imaging of Three-Dimensional Cell Culture on Opaque Bioscaffolds for Tissue Engineering Applications.ACS applied bio materials · 2023Article
- Efficient Generation of Chondrocytes From Bone Marrow-Derived Mesenchymal Stem Cells in a 3D Culture System: Protocol for a Practical Model for Assessing Anti-Inflammatory Therapies.JMIR research protocols · 2023Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors.
Funding
Abstract
From growing cells in spheroids to arranging them on complex engineered scaffolds, three-dimensional cell culture protocols are rapidly expanding and diversifying. While these systems may often improve the physiological relevance of cell culture models, they come with technical challenges, as many of the analytical methods used to characterize traditional two-dimensional (2D) cells must be modified or replaced to be effective. Here we review the advantages and limitations of quantification methods based either on biochemical measurements or microscopy imaging. We focus on the most basic of parameters that one may want to measure, the number of cells. Precise determination of this number is essential for many analytical techniques where measured quantities are only meaningful when normalized to the number of cells (e.g. cytochrome p450 enzyme activity). Thus, accurate measurement of cell number is often a prerequisite to allowing comparisons across different conditions (culturing conditions or drug and treatment screening) or between cells in different spatial states. We note that this issue is often neglected in the literature with little or no information given regarding how normalization was performed, we highlight the pitfalls and complications of quantification and call for more accurate reporting to improve reproducibility.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.