ArticleACS catalysis2022
Deciphering the Reaction Pathway of Mononuclear Iron Enzyme-Catalyzed N≡C Triple Bond Formation in Isocyanide Lipopeptide and Polyketide Biosynthesis.
Article in ACS catalysis, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 25 citations in OpenAlex.
- Molecular Basis of α-Glycine C-H Activation by a Nonheme Fe(II)/2-Oxoglutarate Dioxygenase.Biochemistry · 2026Article
- A highly dynamic mononuclear non-heme iron enzyme for the two-step isonitrile biosynthesis.Nature communications · 2026Article
- Uncovering the Origins of Selectivity in Non-Heme Iron Dioxygenase-Catalyzed Tropolone Biosynthesis.The journal of physical chemistry. B · 2025Article
- Studies of Isonitrile Synthases Help Elucidate the Mechanism of Isonitrile Installation.ACS catalysis · 2025Article
- Copper-chelating natural products.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2025Review
- Biosynthesis of isonitrile lipopeptides.Current opinion in chemical biology · 2024Review
- Biosynthesis of the Unusual Epoxy Isonitrile-Containing Antibiotics Aerocyanidin and Amycomicin.Journal of the American Chemical Society · 2024Article
- Variation in biosynthesis and metal-binding properties of isonitrile-containing peptides produced by Mycobacteria versus Streptomyces.ACS catalysis · 2024Article
- Actinobacterial chalkophores: the biosynthesis of hazimycins.The Journal of antibiotics · 2024Article
- Spectroscopic and computational studies of a bifunctional iron- and 2-oxoglutarate dependent enzyme, AsqJ.Methods in enzymology · 2024Article
- Repurposing Iron- and 2-Oxoglutarate-Dependent Oxygenases to Catalyze Olefin Hydration.Angewandte Chemie (International ed. in English) · 2023Article
- Biosynthesis of Isonitrile Lipopeptide Metallophores from Pathogenic Mycobacteria.Biochemistry · 2023Article
- Five decades of metalloenzymology.The Enzymes · 2023Review
- Probing the Mechanism of Isonitrile Formation by a Non-Heme Iron(II)-Dependent Oxidase/Decarboxylase.Journal of the American Chemical Society · 2022Article
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11 authors at 5 institutions in 2 countries.
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Abstract
Despite the diversity of reactions catalyzed by 2-oxoglutarate-dependent nonheme iron (Fe/2OG) enzymes identified in recent years, only a limited number of these enzymes have been investigated in mechanistic detail. In particular, several Fe/2OG-dependent enzymes capable of catalyzing isocyanide formation have been reported. While the glycine moiety has been identified as a biosynthon for the isocyanide group, how the actual conversion is effected remains obscure. To elucidate the catalytic mechanism, we characterized two previously unidentified (AecA and AmcA) along with two known (ScoE and SfaA) Fe/2OG-dependent enzymes that catalyze N≡C triple bond installation using synthesized substrate analogues and potential intermediates. Our results indicate that isocyanide formation likely entails a two-step sequence involving an imine intermediate that undergoes decarboxylation-assisted desaturation to yield the isocyanide product. Results obtained from the
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