ArticleFrontiers in bioengineering and biotechnology2022
Flexible Terahertz Metamaterial Biosensor for Ultra-Sensitive Detection of Hepatitis B Viral DNA Based on the Metal-Enhanced Sandwich Assay.
Article in Frontiers in bioengineering and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Research Progress of Terahertz Technology in Microbiology.Biosensors · 2026Review
- Interconnected four split rectangular ring resonator flexible metamaterial for microwave sensing application.Scientific reports · 2025Article
- Label-free sensing technology of two Staphylococcus species based on a terahertz metasurface.Biomedical optics express · 2025Article
- A new horizon for neuroscience: terahertz biotechnology in brain research.Neural regeneration research · 2025Article
- Microdetection of Nucleocapsid Proteins via Terahertz Chemical Microscope Using Aptamers.Sensors (Basel, Switzerland) · 2024Article
- CRISPR/Cas12a cleavage triggered nanoflower for fluorescence-free and target amplification-free biosensing of ctDNA in the terahertz frequencies.Biomedical optics express · 2024Article
- Editorial: Rapid detection of fungi, microbial, and viral pathogens based on emerging biosensing technology.Frontiers in bioengineering and biotechnology · 2022Article
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Authors and funding
10 authors.
Funding
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Abstract
The high sensitivity and specificity of terahertz (THz) biosensing are both promising and challenging in DNA sample detection. This study produced and refined a flexible THz MM biosensor for ultrasensitive detection of HBV in clinical serum samples based on a gold magnetic nanoparticle-mediated rolling circle amplification (GMNPs@RCA) sandwich assay under isothermal conditions. Typically, solid-phase RCA reactions mediated by circular padlock probes (PLPs) are triggered under isothermal conditions in the presence of HBV DNA, resulting in long single-stranded DNA (ssDNA) with high fidelity and specificity. Then, the resultant ssDNA was conjugated with detection probes (DPs) immobilized on gold nanoparticles (DP@AuNPs) to form GMNPs-RCA-AuNPs sandwich complexes. The HBV DNA concentrations were quantified by introducing GMNPs-RCA-AuNPs complexes into the metasurface of a flexible THz metamaterial-based biosensor chip and resulting in a red shift of the resonance peak of the THz metamaterials. This biosensor can lead to highly specific and sensitive detection with one-base mismatch discrimination and a limit of detection (LOD) down to 1.27E + 02 IU/ml of HBV DNA from clinical serum samples. The HBV DNA concentration was linearly correlated with the frequency shift of the THz metamaterials within the range of 1.27E + 02∼1.27E + 07 IU/ml, illustrating the applicability and accuracy of our assay in real clinical samples. This strategy constitutes a promising THz sensing method to identify virus DNA. In the future, it is hoped it can assist with pathogen identification and clinical diagnosis.
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