ArticleFrontiers in bioengineering and biotechnology2022
Development of a Chemically Defined Medium for
Article in Frontiers in bioengineering and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed.
- Understanding Osmolality as a Critical Process Parameter in Mammalian Muscle Cell Culture and Biomanufacturing.ACS omega · 2026Review
- Towards Cost-Effective and Sustainable Media Formulations for Terrestrial and Aquatic Cellular Agriculture.Foods (Basel, Switzerland) · 2026Review
- A fingerprint-based polymeric sensing platform for comprehensive quality assessment of complex culture media in cell manufacturing.Chemical science · 2026Article
- Optimization of Serum-Free Culture Medium for Theileria annulata-Transformed Cell Lines: A Comparative Analysis.Acta parasitologica · 2026Article
- L-Carnosine Enhances the Proliferation and Myogenic Differentiation of Yanbian Cattle Skeletal Muscle Satellite Cells for Cultured Meat Production via Activating the Akt/mTOR/P70S6K Signaling Pathway.Food science & nutrition · 2026Article
- Nutritional, Ethical and Ecological Aspects of Cultured Meat with Particular Emphasis on Functional Food Production: A Comprehensive Literature Review.Foods (Basel, Switzerland) · 2026Review
- A small molecule strategy with forskolin and p38 inhibitor for serum-free muscle stem cell expansion.NPJ science of food · 2026Article
- Development of Cell Culture Media for Cultivated Meat Production.Advances in biochemical engineering/biotechnology · 2026Review
- Exploring ethical, sustainable and effective foetal bovine serum alternatives forFrontiers in toxicology · 2026Review
- Animal-derived components in cultivated meat research and their alternatives.NPJ science of food · 2025Review
- Multi-response optimization of defined and undefined serum-free media for enhanced proliferation of immortalized lamb muscle cells.Scientific reports · 2025Article
- Whey-enhanced serum-free medium for bovine myoblast expansion.Food science and biotechnology · 2025Article
- Cloning, expression and functional characterization of prepared bovine, salmon, and cod basic fibroblast growth factor-2.NPJ science of food · 2025Article
- Chick embryo extract-driven culture system enhances chicken muscle satellite cell differentiation and 3D myotube formation.NPJ science of food · 2025Article
- Forty-one degrees celsius enhances proliferation of chicken muscle satellite cells via mechanistic target of rapamycin activation and mitochondrial metabolism.Poultry science · 2025Article
- Dairy byproducts as sustainable alternatives to FCS in 2D and 3D skeletal muscle cell cultures.Bioresources and bioprocessing · 2025Article
- Serum-free cultured meat production by usingiScience · 2025Article
- Enhanced Media Optimize Bovine Myogenesis in 2D and 3D Models for Cultivated Meat Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Low-cost food-grade alternatives for serum albumins in FBS-free cell culture media.Scientific reports · 2025Article
- The Role of Insulin in the Proliferation and Differentiation of Bovine Muscle Satellite (Stem) Cells for Cultured Meat Production.International journal of molecular sciences · 2025Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of fetal bovine serum (FBS) in animal cell culture media is widely spread since it provides a broad spectrum of molecules that are known to support cell attachment and growth. However, the harvest and collection procedures of FBS raise ethical concerns and serum is an ill-defined and expensive component. This is especially problematic when it comes to regulatory approval for food applications like cultured meat. The aim of this study is to develop a chemically defined, cost efficient serum-free and animal-free medium that supports the attachment and expansion of bovine myoblasts while maintaining their differentiation capacity. Bovine satellite cells were harvested and isolated from a fresh sample of skeletal muscle tissue and cultured in planar systems. The efficacy of the tested formulations was assessed with metabolic assays and cell counting techniques. Optical microscopy was used to observe cellular morphology and statistical analysis was applied. Based on a comprehensive literature analysis, a defined serum-free medium (SFM) composition was developed consisting of DMEM/F12 as basal medium, supplemented with L-ascorbic acid 2-phosphate, fibronectin, hydrocortisone, GlutaMAX
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.