Evidence map›Paper›PMID 35992231›Full record

ArticleNational science review2022

A CRISPR/Cas12a-empowered surface plasmon resonance platform for rapid and specific diagnosis of the Omicron variant of SARS-CoV-2.

Zhi Chen, Jingfeng Li, Tianzhong Li, Taojian Fan, Changle Meng, Chaozhou Li, Jianlong Kang, Luxiao Chai, Yabin Hao, Yuxuan Tang and 13 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in National science review, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.

0numbers the graph read from it
0cells of the map it votes in
69citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

69 citing papers in PubMed.

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9 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

23 authors.

Zhi ChenShenzhen Engineering Laboratory of Phosphorene and Optoelectronics; International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education; Shenzhen Institute of Translational Medicine; Department of Otolaryngology, Shenzhen Second People's Hospital; the First Affiliated Hospital; Institute of Microscale Optoelectronics, Shenzhen University, Shenzhen 518060, China.
Jingfeng LiShenzhen Engineering Laboratory of Phosphorene and Optoelectronics; International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education; Shenzhen Institute of Translational Medicine; Department of Otolaryngology, Shenzhen Second People's Hospital; the First Affiliated Hospital; Institute of Microscale Optoelectronics, Shenzhen University, Shenzhen 518060, China.
Tianzhong LiShenzhen Engineering Laboratory of Phosphorene and Optoelectronics; International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education; Shenzhen Institute of Translational Medicine; Department of Otolaryngology, Shenzhen Second People's Hospital; the First Affiliated Hospital; Institute of Microscale Optoelectronics, Shenzhen University, Shenzhen 518060, China.
Taojian FanShenzhen Engineering Laboratory of Phosphorene and Optoelectronics; International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education; Shenzhen Institute of Translational Medicine; Department of Otolaryngology, Shenzhen Second People's Hospital; the First Affiliated Hospital; Institute of Microscale Optoelectronics, Shenzhen University, Shenzhen 518060, China.
Changle MengShenzhen Engineering Laboratory of Phosphorene and Optoelectronics; International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education; Shenzhen Institute of Translational Medicine; Department of Otolaryngology, Shenzhen Second People's Hospital; the First Affiliated Hospital; Institute of Microscale Optoelectronics, Shenzhen University, Shenzhen 518060, China.
Chaozhou LiShenzhen Engineering Laboratory of Phosphorene and Optoelectronics; International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education; Shenzhen Institute of Translational Medicine; Department of Otolaryngology, Shenzhen Second People's Hospital; the First Affiliated Hospital; Institute of Microscale Optoelectronics, Shenzhen University, Shenzhen 518060, China.
Jianlong KangShenzhen Engineering Laboratory of Phosphorene and Optoelectronics; International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education; Shenzhen Institute of Translational Medicine; Department of Otolaryngology, Shenzhen Second People's Hospital; the First Affiliated Hospital; Institute of Microscale Optoelectronics, Shenzhen University, Shenzhen 518060, China.
Luxiao ChaiShenzhen Engineering Laboratory of Phosphorene and Optoelectronics; International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education; Shenzhen Institute of Translational Medicine; Department of Otolaryngology, Shenzhen Second People's Hospital; the First Affiliated Hospital; Institute of Microscale Optoelectronics, Shenzhen University, Shenzhen 518060, China.
Yabin HaoShenzhen Engineering Laboratory of Phosphorene and Optoelectronics; International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education; Shenzhen Institute of Translational Medicine; Department of Otolaryngology, Shenzhen Second People's Hospital; the First Affiliated Hospital; Institute of Microscale Optoelectronics, Shenzhen University, Shenzhen 518060, China.
Yuxuan TangShenzhen Engineering Laboratory of Phosphorene and Optoelectronics; International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education; Shenzhen Institute of Translational Medicine; Department of Otolaryngology, Shenzhen Second People's Hospital; the First Affiliated Hospital; Institute of Microscale Optoelectronics, Shenzhen University, Shenzhen 518060, China.
Omar A Al-HartomyDepartment of Physics, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
Swelm WagehDepartment of Physics, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
Abdullah G Al-SehemiResearch Center for Advanced Materials Science (RCAMS), King Khalid University, Abha 61413, Saudi Arabia.
Zhiguang LuoZhongmin (Shenzhen) Intelligent Ecology Co. Ltd, Shenzhen 518055, China.
Jiangtian YuShenzhen International Institute for Biomedical Research, Shenzhen 518116, China.
Yonghong ShaoKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China.
Defa LiDepartment of Laboratory Medicine, Shenzhen Children's Hospital, Shenzhen 518038, China.
Shuai FengOptoelectronics Research Center, School of Science, Minzu University of China, Beijing 100081, China.
William J LiuNHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Yaqing HeInstitute of Pathogenic Organism, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
Xiaopeng MaRespiratory Department , Shenzhen Children's Hospital, Shenzhen 518038, China.
Zhongjian XieInstitute of Pediatrics, Shenzhen Children's Hospital, Shenzhen 518038, China.
Han ZhangShenzhen Engineering Laboratory of Phosphorene and Optoelectronics; International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education; Shenzhen Institute of Translational Medicine; Department of Otolaryngology, Shenzhen Second People's Hospital; the First Affiliated Hospital; Institute of Microscale Optoelectronics, Shenzhen University, Shenzhen 518060, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The outbreak of the COVID-19 pandemic was partially due to the challenge of identifying asymptomatic and presymptomatic carriers of the virus, and thus highlights a strong motivation for diagnostics with high sensitivity that can be rapidly deployed. On the other hand, several concerning SARS-CoV-2 variants, including Omicron, are required to be identified as soon as the samples are identified as 'positive'. Unfortunately, a traditional PCR test does not allow their specific identification. Herein, for the first time, we have developed MOPCS (Methodologies of Photonic CRISPR Sensing), which combines an optical sensing technology-surface plasmon resonance (SPR) with the 'gene scissors' clustered regularly interspaced short palindromic repeat (CRISPR) technique to achieve both high sensitivity and specificity when it comes to measurement of viral variants. MOPCS is a low-cost, CRISPR/Cas12a-system-empowered SPR gene-detecting platform that can analyze viral RNA, without the need for amplification, within 38 min from sample input to results output, and achieve a limit of detection of 15 fM. MOPCS achieves a highly sensitive analysis of SARS-CoV-2, and mutations appear in variants B.1.617.2 (Delta), B.1.1.529 (Omicron) and BA.1 (a subtype of Omicron). This platform was also used to analyze some recently collected patient samples from a local outbreak in China, identified by the Centers for Disease Control and Prevention. This innovative CRISPR-empowered SPR platform will further contribute to the fast, sensitive and accurate detection of target nucleic acid sequences with single-base mutations.

Indexed as

CRISPROmicron variantSARS-CoV-2surface plasmon resonance

Identifiers

PMID35992231
PMCPMC9385456

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