Evidence map›Paper›PMID 35991161›Full record

ArticleBio-protocol2022

A β-glucuronidase (GUS) Based Bacterial Competition Assay to Assess Fine Differencesin Fitness during Plant Infection.

Julien S Luneau, Laurent D Noël, Emmanuelle Lauber, Alice Boulanger

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Julien S LuneauLaboratory of Plant-Microbes Interactions, Université de Toulouse, INRAE, CNRS, UPS, Castanet-Tolosan, France.
Laurent D NoëlLaboratory of Plant-Microbes Interactions, Université de Toulouse, INRAE, CNRS, UPS, Castanet-Tolosan, France.
Emmanuelle LauberLaboratory of Plant-Microbes Interactions, Université de Toulouse, INRAE, CNRS, UPS, Castanet-Tolosan, France.
Alice BoulangerLaboratory of Plant-Microbes Interactions, Université de Toulouse, INRAE, CNRS, UPS, Castanet-Tolosan, France.
Laboratoire des Interactions Plantes Micro-Organismes · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Competition assays are a simple phenotyping strategy that confront two bacterial strains to evaluate their relative fitness. Because they are more accurate than single-strain growth assays, competition assays can be used to highlight slight differences that would not otherwise be detectable. In the frame of host-pathogens interactions, they can be very useful to study the contribution of individual bacterial genes to bacterial fitness and lead to the identification of new adaptive traits. Here, we describe how to perform such competition assays by taking the example of the model phytopathogenic bacterium

Indexed as

Brassica oleraceaCauliflowerCompetition assayCompetitive IndexFitnessGUSMesophyllXanthomonas

Identifiers

PMID35991161
PMCPMC9382408
OpenAlexW4242302219

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.