Evidence map›Paper›PMID 35990905›Full record

ArticlePeerJ2022

The role of autophagy in high-fat diet-induced insulin resistance of adipose tissues in mice.

Yovita Permata Budi, Yi-Hsuan Li, Chien Huang, Mu-En Wang, Yi-Chun Lin, De-Shien Jong, Chih-Hsien Chiu, Yi-Fan Jiang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PeerJ, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.9field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 12 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

Yovita Permata Budi *Graduate Institute of Molecular and Comparative Pathobiology, National Taiwan University, Taipei, Taiwan.
Yi-Hsuan Li *Department of Animal Science and Technology, National Taiwan University, Taipei, Taiwan.
Chien HuangDepartment of Animal Science and Technology, National Taiwan University, Taipei, Taiwan.
Mu-En WangDepartment of Pathology, Duke University, North Carolina, Durham, United States of America.
Yi-Chun LinDepartment of Animal Science, National Chung Hsing University, Taichung, Taichung, Taiwan.
De-Shien JongDepartment of Animal Science and Technology, National Taiwan University, Taipei, Taiwan.
Chih-Hsien ChiuDepartment of Animal Science and Technology, National Taiwan University, Taipei, Taiwan.
Yi-Fan JiangGraduate Institute of Molecular and Comparative Pathobiology, National Taiwan University, Taipei, Taiwan.
National Taiwan University of Science and Technology · TWNational Taiwan University · TWDuke University · USNational Chung Hsing University · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Aims: Studies have observed changes in autophagic flux in the adipose tissue of type 2 diabetes patients with obesity. However, the role of autophagy in obesity-induced insulin resistance is unclear. We propose to confirm the effect of a high-fat diet (HFD) on autophagy and insulin signaling transduction from adipose tissue to clarify whether altered autophagy-mediated HFD induces insulin resistance, and to elucidate the possible mechanisms in autophagy-regulated adipose insulin sensitivity. Methods: Eight-week-old male C57BL/6 mice were fed with HFD to confirm the effect of HFD on autophagy and insulin signaling transduction from adipose tissue. Differentiated 3T3-L1 adipocytes were treated with 1.2 mM fatty acids (FAs) and 50 nM Bafilomycin A1 to determine the autophagic flux. 2.5 mg/kg body weight dose of Chloroquine (CQ) in PBS was locally injected into mouse epididymal adipose (10 and 24 h) and 40 µM of CQ to 3T3-L1 adipocytes for 24 h to evaluate the role of autophagy in insulin signaling transduction. Results: The HFD treatment resulted in a significant increase in SQSTM1/p62, Rubicon expression, and C/EBP homologous protein (CHOP) expression, yet the insulin capability to induce Akt (Ser473) and GSK3 Conclusion: Long-term high-fat diet promotes insulin resistance, late-stage autophagy inhibition, ER stress, and apoptosis in adipose tissue. Autophagy suppression may not affect insulin signaling transduction

Indexed as

Diabetes Mellitus, Type 2Insulin ResistanceAdipose TissueAnimalsAutophagyDiet, High-FatInsulinMacrolidesMaleMiceMice, Inbred C57BLObesityProto-Oncogene Proteins c-aktbafilomycin A1InsulinMacrolidesProto-Oncogene Proteins c-aktApoptosisAutophagyER stressHigh-fat dietInsulin resistanceObesity

Identifiers

PMID35990905
PMCPMC9387522
OpenAlexW4291448414

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.