Evidence map›Paper›PMID 35989594›Full record

ArticleThe FEBS journal2023

Regulation of the pyrimidine biosynthetic pathway by lysine acetylation of E. coli OPRTase.

Gema Lozano-Terol, Julia Gallego-Jara, Rosa Alba Sola-Martínez, Álvaro Ortega, Adrián Martínez Vivancos, Manuel Cánovas Díaz, Teresa de Diego Puente

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The FEBS journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Gema Lozano-TerolDepartment of Biochemistry and Molecular Biology and Immunology (B), Faculty of Chemistry, University of Murcia, Spain.ORCID 0000-0001-7611-4459
Julia Gallego-JaraDepartment of Biochemistry and Molecular Biology and Immunology (B), Faculty of Chemistry, University of Murcia, Spain.
Rosa Alba Sola-MartínezDepartment of Biochemistry and Molecular Biology and Immunology (B), Faculty of Chemistry, University of Murcia, Spain.
Álvaro OrtegaDepartment of Biochemistry and Molecular Biology and Immunology (B), Faculty of Chemistry, University of Murcia, Spain.
Adrián Martínez VivancosDepartment of Biochemistry and Molecular Biology and Immunology (B), Faculty of Chemistry, University of Murcia, Spain.
Manuel Cánovas DíazDepartment of Biochemistry and Molecular Biology and Immunology (B), Faculty of Chemistry, University of Murcia, Spain.
Teresa de Diego PuenteDepartment of Biochemistry and Molecular Biology and Immunology (B), Faculty of Chemistry, University of Murcia, Spain.ORCID 0000-0003-3501-5483
Universidad de Murcia · ES

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The de novo pyrimidine biosynthesis pathway is an important route due to the relevance of its products, its implications in health and its conservation among organisms. Here, we investigated the regulation by lysine acetylation of this pathway. To this aim, intracellular and extracellular metabolites of the route were quantified, revealing a possible blockage of the pathway by acetylation of the OPRTase enzyme (orotate phosphoribosyltransferase). Chemical acetylation of OPRTase by acetyl-P involved a decrease in enzymatic activity. To test the effect of acetylation in this enzyme, K26 and K103 residues were selected to generate site-specific acetylated proteins. Several differences were observed in kinetic parameters, emphasizing that the k

Indexed as

Escherichia coliLysineAcetylationBiosynthetic PathwaysPyrimidinesLysinePyrimidinesE. colilysine acetylationOPRTasepyrimidine biosynthesis pathwayregulation

Identifiers

PMID35989594
PMCPMC10087573
OpenAlexW4292572968

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.