Evidence map›Paper›PMID 35989549›Full record

ArticleThe FEBS journal2023

Single-molecule tracking of perfringolysin O assembly and membrane insertion uncoupling.

Michael J T Senior, Carina Monico, Eve E Weatherill, Robert J Gilbert, Alejandro P Heuck, Mark I Wallace

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The FEBS journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.8field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 11 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 2 countries.

Michael J T SeniorChemistry Research Laboratory, Department of Chemistry, University of Oxford, UK.ORCID 0000-0002-9064-7769
Carina MonicoChemistry Research Laboratory, Department of Chemistry, University of Oxford, UK.
Eve E WeatherillChemistry Research Laboratory, Department of Chemistry, University of Oxford, UK.
Robert J GilbertDivision of Structural Biology, Wellcome Centre for Human Genetics, University of Oxford, UK.
Alejandro P HeuckDepartments of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, MA, USA.
Mark I WallaceDepartment of Chemistry, King's College London, UK.ORCID 0000-0002-5692-8313
King's College London · GBCentre for Human Genetics · GBUniversity of Massachusetts Amherst · USUniversity of Oxford · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We exploit single-molecule tracking and optical single channel recording in droplet interface bilayers to resolve the assembly pathway and pore formation of the archetypical cholesterol-dependent cytolysin nanopore, Perfringolysin O. We follow the stoichiometry and diffusion of Perfringolysin O complexes during assembly with 60 ms temporal resolution and 20 nm spatial precision. Our results suggest individual nascent complexes can insert into the lipid membrane where they continue active assembly. Overall, these data support a model of stepwise irreversible assembly dominated by monomer addition, but with infrequent assembly from larger partial complexes.

Indexed as

Bacterial ToxinsCell MembraneCholesterolHemolysin ProteinsBacterial ToxinsCholesterolClostridium perfringens theta-toxinHemolysin ProteinsassemblyDIBfluorescencePFOphotobleachingsingle molecule

Identifiers

PMID35989549
PMCPMC10086847
OpenAlexW4292553145

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.