Evidence map›Paper›PMID 35989496›Full record

ArticleJournal of clinical laboratory analysis2022

Upregulation of hsa_circ_0004812 promotes COVID-19 cytokine storm via hsa-miR-1287-5p/IL6R, RIG-I axis.

Elham Mohammadisoleimani, Zahra Firoozi, Mohammad Mehdi Naghizadeh, Ali Ghanbari Asad, Babak Pezeshki, Yousef Gholampour, Ebrahim Mirzaei, Mohamadhosain Simin, Mahsa Saliani, Ali Moravej and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of clinical laboratory analysis, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.9field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 2 countries.

Elham MohammadisoleimaniDepartment of Medical Biotechnology, Fasa University of Medical Sciences, Fasa, Iran.ORCID https://orcid.org/0000-0002-3950-3102
Zahra FirooziDepartment of Medical Genetics, Fasa University of Medical Sciences, Fasa, Iran.
Mohammad Mehdi NaghizadehNoncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran.ORCID https://orcid.org/0000-0001-5562-103X
Ali Ghanbari AsadDepartment of Medical Biotechnology, Fasa University of Medical Sciences, Fasa, Iran.ORCID https://orcid.org/0000-0001-6423-4680
Babak PezeshkiNoncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran.
Yousef GholampourDepartment of Internal Medicine, Fasa University of Medical Sciences, Fasa, Iran.
Ebrahim MirzaeiNoncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran.
Mohamadhosain SiminSchool of Medicine, Fasa University of Medical Sciences, Fasa, Iran.
Mahsa SalianiInstitute of Biochemistry and Molecular Biology II, Medical Faculty, Heinrich Heine University, Düsseldorf, Germany.
Ali MoravejDepartment of Immunology, Fasa University of Medical Sciences, Fasa, Iran.
Zolfaghar ArmoonDepartment of Internal Medicine, Fasa University of Medical Sciences, Fasa, Iran.
Yaser MansooriDepartment of Medical Genetics, Fasa University of Medical Sciences, Fasa, Iran.ORCID https://orcid.org/0000-0002-9101-5552
Fasa University of Medical Sciences · IRHeinrich Heine University Düsseldorf · DE

Funding

Fasa University of Medical Sciences 99157
6 · The paper itself

Abstract

backgroundSARS-CoV-2 is one of the most contagious viruses in the Coronaviridae (CoV) family, which has become a pandemic. The aim of this study is to understand more about the role of hsa_circ_0004812 in the SARS-CoV-2 related cytokine storm and its associated molecular mechanisms. MATERIALS AND

methodscDNA synthesis was performed after total RNA was extracted from the peripheral blood mononuclear cells (PBMC) of 46 patients with symptomatic COVID-19, 46 patients with asymptomatic COVID-19, and 46 healthy controls. The expression levels of hsa_circ_0004812, hsa-miR-1287-5p, IL6R, and RIG-I were determined using qRT-PCR, and the potential interaction between these molecules was confirmed by bioinformatics tools and correlation analysis.

resultshsa_circ_0004812, IL6R, and RIG-I are expressed higher in the severe symptom group compared with the negative control group. Also, the relative expression of these genes in the asymptomatic group is lower than in the severe symptom group. The expression level of hsa-miR-1287-5p was positively correlated with symptoms in patients. The results of the bioinformatics analysis predicted the sponging effect of hsa_circ_0004812 as a competing endogenous RNA on hsa-miR-1287-5p. Moreover, there was a significant positive correlation between hsa_circ_0004812, RIG-I, and IL-6R expressions, and also a negative expression correlation between hsa_circ_0004812 and hsa-miR-1287-5p and between hsa-miR-1287-5p, RIG-I, and IL-6R.

conclusionThe results of this in-vitro and in silico study show that hsa_circ_0004812/hsa-miR-1287-5p/IL6R, RIG-I can play an important role in the outcome of COVID-19.

Indexed as

COVID-19MicroRNAsCell ProliferationCytokine Release SyndromeDNA, ComplementaryHumansLeukocytes, MononuclearReceptors, Cell SurfaceReceptors, Interleukin-6RNA, CircularSARS-CoV-2Up-RegulationDNA, ComplementaryIL6R protein, humanMicroRNAsMIRN1287 microRNA, humanReceptors, Cell SurfaceReceptors, Interleukin-6RNA, Circularcytokine stormhsa_circ_0004812hsa-miR-1287-5pSARS-CoV-2sponging effect

Identifiers

PMID35989496
PMCPMC9538103
OpenAlexW4292560291

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.