ArticleNature biomedical engineering2022
Diagnosis of paediatric tuberculosis by optically detecting two virulence factors on extracellular vesicles in blood samples.
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Article in Nature biomedical engineering, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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43citing papers in PubMed
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Who cites it
43 citing papers in PubMed, 75 citations in OpenAlex.
- The Proteome of Plasma Extracellular Vesicles in Latent and Active Tuberculosis Reveals Phase-Specific Composition and Immunometabolic Insights.FASEB bioAdvances · 2026Article
- Hijacking Host Communication: The Central Role of Extracellular Vesicles in Infectious Disease Pathogenesis.Cells · 2026Review
- Role of Exosomes During Mycobacterium Tuberculosis Infection: A Double-Edged Sword.Biomedicines · 2026Review
- Neonatal small extracellular vesicle-loaded GelNB hydrogel reprograms the vascular-immune microenvironment for spinal cord injury repair.Bioactive materials · 2026Article
- iEVIP: a dual-inspired intelligent platform for smart response extracellular vesicle isolation and single-vesicle profiling.Nature communications · 2026Article
- Bilateral SERS-Microneedle Patch for Co-Diagnosis of Diabetes Mellitus and Tuberculosis Comorbidity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Extracellular Vesicles of Mycobacterium tuberculosis Serve as a Virulence Factor - Current Research Applications.Current microbiology · 2026Review
- The 'sugar' side of extracellular vesicle-glycome: a panorama from basic characteristics, deciphering technologies, functions, to applications.Journal of nanobiotechnology · 2026Review
- Evaluation of non-sputum-based diagnostics for pediatric tuberculosis: the Pediatric TB Diagnostic (PDTBDx) cohort protocol.medRxiv : the preprint server for health sciences · 2026Article
- PTBD: a machine learning-based non-invasive diagnostic model for pulmonary tuberculosis using large-scale blood transcriptomes.BMC biology · 2026Article
- Amplifying metabolic profiling of extracellular vesicle dynamics with ACTIVITY.Nature communications · 2026Article
- Profiling of Integrin Isoforms on Lung-Tropic Exosomes by Spectrally and Kinetically Multiplexed Single-Molecule Imaging.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Downregulated Plasma-Derived Extracellular Vesicle MicroRNAs as Novel Biomarkers for Enhanced Tuberculosis Diagnosis.Journal of immunology research · 2026Article
- Salivary exosomalFrontiers in cellular and infection microbiology · 2026Article
- Alterations in the Immune Response in Individuals with Latent Tuberculosis Infection.Pathogens (Basel, Switzerland) · 2025Review
- Tuberculous meningitis alters the proteomic landscape of brain-derived extracellular vesicles.bioRxiv : the preprint server for biology · 2025Article
- Lab-in-a-cartridge for real-time detection of tuberculosis via precise measurement of urinary lipoarabinomannan.Nature communications · 2025Article
- Emerging technologies towards extracellular vesicles large-scale production.Bioactive materials · 2025Review
- Extracellular Vesicles for Clinical Diagnostics: From Bulk Measurements to Single-Vesicle Analysis.ACS nano · 2025Review
- An energy-embodied paralleled liquid manipulation for equipment-free, quantitative multiplexed liver function monitoring.Science advances · 2025Article
Corrections and comments
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Authors and funding
37 authors at 12 institutions in 3 countries.
Wenshu ZhengCenter for Cellular and Molecular Diagnostics, Tulane University School of Medicine, New Orleans, LA, USA.
Sylvia M LaCourseDepartment of Medicine, Division of Allergy and Infectious Diseases, University of Washington, Seattle, WA, USA.
Bofan SongJames C. Wyant College of Optical Sciences, The University of Arizona, Tucson, AZ, USA.
Dhiraj Kumar SinghSouthwest National Primate Research Center, Texas Biomedical Research Institute, San Antonio, TX, USA.ORCID http://orcid.org/0000-0001-5507-1197
Mayank KhannaDepartment of Microbiology, Immunology and Parasitology, LSU Health Sciences Center, New Orleans, LA, USA.
Juan OlivoO&M Medical School (O&Med), Santo Domingo, Dominican Republic.
Joshua SternDepartment of Global Health, University of Washington, Seattle, WA, USA.
Jaclyn N EscuderoDepartment of Global Health, University of Washington, Seattle, WA, USA.
Carlos VergaraO&M Medical School (O&Med), Santo Domingo, Dominican Republic.
Fangfang ZhangVirginia G. Piper Biodesign Center for Personalized Diagnostics, The Biodesign Institute, Arizona State University, Tempe, AZ, USA.
Shaobai LiJames C. Wyant College of Optical Sciences, The University of Arizona, Tucson, AZ, USA.
Shu WangCenter for Cellular and Molecular Diagnostics, Tulane University School of Medicine, New Orleans, LA, USA.
Lisa M CranmerDepartment of Pediatrics, Division of Pediatric Infectious Diseases, Emory School of Medicine, Atlanta, GA, USA.ORCID http://orcid.org/0000-0002-6938-9195
Zhen HuangCenter for Cellular and Molecular Diagnostics, Tulane University School of Medicine, New Orleans, LA, USA.ORCID http://orcid.org/0000-0002-3990-7350
Christine M BojanowskiSection of Pulmonary Diseases, Tulane University School of Medicine, New Orleans, LA, USA.
Duran BaoCenter for Cellular and Molecular Diagnostics, Tulane University School of Medicine, New Orleans, LA, USA.
Irene NjugunaDepartment of Global Health, University of Washington, Seattle, WA, USA.
Yating XiaoCenter for Cellular and Molecular Diagnostics, Tulane University School of Medicine, New Orleans, LA, USA.
Dalton C WamalwaDepartment of Global Health, University of Washington, Seattle, WA, USA.
Duc T NguyenDepartment of Pathology and Genomic Medicine, Houston Methodist, Houston, TX, USA.ORCID http://orcid.org/0000-0002-5059-4404
Li YangCenter for Cellular and Molecular Diagnostics, Tulane University School of Medicine, New Orleans, LA, USA.
Elizabeth Maleche-ObimboDepartment of Global Health, University of Washington, Seattle, WA, USA.
Nhung NguyenNational Lung Hospital, Ha Noi, Vietnam.
Lili ZhangCenter for Cellular and Molecular Diagnostics, Tulane University School of Medicine, New Orleans, LA, USA.
Ha PhanCenter for Promotion of Advancement of Society (CPAS), Ha Noi, Vietnam.
Jia FanCenter for Cellular and Molecular Diagnostics, Tulane University School of Medicine, New Orleans, LA, USA.
Bo NingCenter for Cellular and Molecular Diagnostics, Tulane University School of Medicine, New Orleans, LA, USA.
Chenzhong LiCenter for Cellular and Molecular Diagnostics, Tulane University School of Medicine, New Orleans, LA, USA.
Christopher J LyonCenter for Cellular and Molecular Diagnostics, Tulane University School of Medicine, New Orleans, LA, USA.
Edward A GravissDepartment of Pathology and Genomic Medicine, Houston Methodist, Houston, TX, USA.ORCID http://orcid.org/0000-0003-1024-5813
Grace John-StewartDepartment of Medicine, Division of Allergy and Infectious Diseases, University of Washington, Seattle, WA, USA.ORCID http://orcid.org/0000-0002-4301-1573
Charles D MitchellDepartment of Pediatrics, Division of Infectious Diseases and Immunology, University of Miami Miller School of Medicine, Batchelor Children's Research Institute, Miami, FL, USA.
Alistair J RamsayDepartment of Microbiology, Immunology and Parasitology, LSU Health Sciences Center, New Orleans, LA, USA.ORCID http://orcid.org/0000-0002-3506-4544
Deepak KaushalSouthwest National Primate Research Center, Texas Biomedical Research Institute, San Antonio, TX, USA.
Rongguang LiangJames C. Wyant College of Optical Sciences, The University of Arizona, Tucson, AZ, USA.
Eddy Pérez-ThenO&M Medical School (O&Med), Santo Domingo, Dominican Republic.
Tony Y HuCenter for Cellular and Molecular Diagnostics, Tulane University School of Medicine, New Orleans, LA, USA. tonyhu@tulane.edu.ORCID http://orcid.org/0000-0001-7255-5409
Tulane University · USUniversity of Washington · USUniversity of Arizona · USHouston Methodist · USLouisiana State University Health Sciences Center New Orleans · USTexas Biomedical Research Institute · USUniversity of Nairobi · KEArizona State University · USChildren’s Institute · USEmory University · USHanoi Lung Hospital · VNUniversity of California, San Francisco · US
Funding
Tulane NPRC SPF Sheltered Outdoor Enclosure ExpansionP51OD011104 · OD · TULANE UNIVERSITY OF LOUISIANA · PI L Lee HAMM · 2012 to 2026
$142.4MThe Southwest National Primate Research Center Supplement- Infrastructure improvements of ABSL2 holding areasP51OD011133 · OD · TEXAS BIOMEDICAL RESEARCH INSTITUTE · PI Larry S. Schlesinger · 2012 to 2026
$129.7MTracking & Evaluation CoreU54GM104940 · NIGMS · LSU PENNINGTON BIOMEDICAL RESEARCH CTR · PI JUDD E SHELLITO · 2012 to 2026
$69.1MViral Testing CoreU42OD010442 · OD · TEXAS BIOMEDICAL RESEARCH INSTITUTE · PI Deepak Kaushal, Kathryn Shelton · 2012 to 2026
$35.6MUrgent Versus Post-Stabilization ART in HIV+ Children with Severe Co-infectionsR01HD023412 · NICHD · UNIVERSITY OF WASHINGTON · PI Grace C. John-Stewart, DALTON CHEKOKO WAMALWA · 1987 to 2026
$12.2MROLE OF INDUCIBLE BRONCHUS ASSOCIATED LYMPHOID TISSUE IN LATENT TUBERCULOSISR01AI111914 · NIAID · WASHINGTON UNIVERSITY · PI KAUSHAL, DEEPAK, KHADER, SHABAANA A. · 2015 to 2025
$11.1MNoncoding RNA Biomarkers for Noninvasive and Early Detection of Pancreatic CancerU01CA214254 · NCI · BECKMAN RESEARCH INSTITUTE/CITY OF HOPE · PI Ajay Goel, DANIEL D VON HOFF · 2017 to 2026
$8.9MMyeloid and lymphoid immunity-specific mechanisms of IDO mediated immunosuppression and its reversal, during TBR01AI134245 · NIAID · TULANE UNIVERSITY OF LOUISIANA · PI Smriti Mehra · 2018 to 2026
$6.2MImmune Correlates of Protection from TBR01AI134240 · NIAID · TULANE UNIVERSITY OF LOUISIANA · PI KAUSHAL, DEEPAK · 2017 to 2022
$4.8MDirect quantitation of the circulating Mtb-peptidome for pediatric TB managementR01HD090927 · NICHD · TULANE UNIVERSITY OF LOUISIANA · PI Christopher J Lyon, Charles DeBeaux Mitchell · 2017 to 2026
$4.5MMtb and HIV/SIV antigen peptide signatures as blood biomarkers to detect early infection to active disease in young children and NHPR01HD103511 · NICHD · TULANE UNIVERSITY OF LOUISIANA · PI LYON, CHRISTOPHER J · 2021 to 2025
$3.7MM. tuberculosis exosome detection for pediatric TB diagnosisR01AI162152 · NIAID · UNIVERSITY OF WASHINGTON · PI LACOURSE, SYLVIA · 2021 to 2025
$3.3MNCI NIH HHS U01 CA214254NCI NIH HHS U01 CA252965NIAID NIH HHS K23 AI120793NIAID NIH HHS K23 AI143479NIAID NIH HHS R01 AI111914NIAID NIH HHS R01 AI113725NIAID NIH HHS R01 AI122932NIAID NIH HHS R01 AI134240NIAID NIH HHS R01 AI134245NIAID NIH HHS R01 AI144168NIAID NIH HHS R01 AI162152NIAID NIH HHS R01 AI175618NIAID NIH HHS R03 AI140977NIAID NIH HHS R21 AI143341NICHD NIH HHS R01 HD023412NICHD NIH HHS R01 HD090927NICHD NIH HHS R01 HD103511NIGMS NIH HHS U54 GM104940NIH HHS P51 OD011104NIH HHS P51 OD011133NIH HHS U42 OD010442NINDS NIH HHS R21 NS130542
Abstract
Sensitive and specific blood-based assays for the detection of pulmonary and extrapulmonary tuberculosis would reduce mortality associated with missed diagnoses, particularly in children. Here we report a nanoparticle-enhanced immunoassay read by dark-field microscopy that detects two Mycobacterium tuberculosis virulence factors (the glycolipid lipoarabinomannan and its carrier protein) on the surface of circulating extracellular vesicles. In a cohort study of 147 hospitalized and severely immunosuppressed children living with HIV, the assay detected 58 of the 78 (74%) cases of paediatric tuberculosis, 48 of the 66 (73%) cases that were missed by microbiological assays, and 8 out of 10 (80%) cases undiagnosed during the study. It also distinguished tuberculosis from latent-tuberculosis infections in non-human primates. We adapted the assay to make it portable and operable by a smartphone. With further development, the assay may facilitate the detection of tuberculosis at the point of care, particularly in resource-limited settings.
Indexed as
Extracellular VesiclesMycobacterium tuberculosisTuberculosisAnimalsCohort StudiesHumansVirulence FactorsVirulence Factors
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