ArticleCommunications biology2022
Single-cell transcriptomics and cell-specific proteomics reveals molecular signatures of sleep.
Article in Communications biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.
What it found
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Who cites it
34 citing papers in PubMed, 1 synthesis or guideline pooled it, 49 citations in OpenAlex.
- Association between sleep duration during pregnancy and gestational diabetes mellitus: a systematic review and meta-analysis.Frontiers in medicine · 2024Pooled it
- Sleep Deprivation and Neuronal Hyperexcitation Share Transcriptomic Signatures.Neuropsychopharmacology reports · 2026Article
- Peripheral and central immune features in insomnia: Integrative bulk and single-cell transcriptomic analyses reveal core hub genes and candidate compounds.Functional & integrative genomics · 2026Article
- A Age Related Vascular Senescence: Mystery of Blood-brain Barrier Dysfunction in Neurodegeneration.Molecular neurobiology · 2026Review
- Hippocampal Glutamatergic Hyperactivation Mediates High-Loading Intensity of Exercise-Induced Cognitive Deficits Via HPC-mPFC Circuit Dysfunction.CNS neuroscience & therapeutics · 2026Article
- A multi-center cross-platform single-cell multimodal atlas of the mouse cerebral cortex.Scientific data · 2026Article
- Sleep regulates drug seeking and relapse - a perspective.Molecular psychiatry · 2026Review
- Sleep regulates hepatic neutrophil trafficking by modulating uric acid metabolism via sympathetic nervous system activity.bioRxiv : the preprint server for biology · 2026Article
- Neuroimaging subtypes of adolescent sleep insufficiency stratify natural short sleepers from comorbidity or environment driven insufficiency.Nature communications · 2026Article
- State-Dependent Remodeling of Astrocytic Proteome and Phosphorylation Signaling Networks Across Wake, Sleep, and General Anesthesia.International journal of molecular sciences · 2026Article
- Cell-specific effects of acute sleep deprivation on transcriptomic signatures of non-neuronal cells in the mouse hippocampus and prefrontal cortex.bioRxiv : the preprint server for biology · 2026Article
- Chronic sleep restriction activates complement and coagulation cascades: a molecular link to accelerated brain aging.Npj biological timing and sleep · 2026Article
- Editorial: Multiomics advances: bridging gaps in understanding intricate biological systems.Frontiers in plant science · 2026Article
- Astrocyte-blood-brain barrier crosstalk in cerebral small vessel disease: linking barrier dysfunction to neurovascular failure.Frontiers in neurology · 2026Review
- Article
- An integrative analysis of cell-specific transcriptomics and nuclear proteomics of sleep-deprived mouse cerebral cortex.Scientific reports · 2025Article
- Review
- Chronic sleep curtailment expediates brain aging by activating the complement and coagulation cascades in mice.bioRxiv : the preprint server for biology · 2025Article
- Revisiting brain gene expression changes and protein modifications tracking homeostatic sleep pressure.Npj biological timing and sleep · 2025Review
- Alterations in Circadian Rhythms, Sleep, and Physical Activity in COVID-19: Mechanisms, Interventions, and Lessons for the Future.Molecular neurobiology · 2024Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 3 countries.
Funding
Abstract
Every day, we sleep for a third of the day. Sleep is important for cognition, brain waste clearance, metabolism, and immune responses. The molecular mechanisms governing sleep are largely unknown. Here, we used a combination of single-cell RNA sequencing and cell-type-specific proteomics to interrogate the molecular underpinnings of sleep. Different cell types in three important brain regions for sleep (brainstem, cortex, and hypothalamus) exhibited diverse transcriptional responses to sleep need. Sleep restriction modulates astrocyte-neuron crosstalk and sleep need enhances expression of specific sets of transcription factors in different brain regions. In cortex, we also interrogated the proteome of two major cell types: astrocytes and neurons. Sleep deprivation differentially alters the expression of proteins in astrocytes and neurons. Similarly, phosphoproteomics revealed large shifts in cell-type-specific protein phosphorylation. Our results indicate that sleep need regulates transcriptional, translational, and post-translational responses in a cell-specific manner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.