Evidence map›Paper›PMID 35986080›Full record

ArticleScientific reports2022

Altered sialidase expression in human myeloid cells undergoing apoptosis and differentiation.

Sang W Hyun, Chiguang Feng, Anguo Liu, Erik P Lillehoj, Rossana Trotta, Tami J Kingsbury, Antonino Passaniti, Katerina N Lugkey, Sitara Chauhan, John F Cipollo and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.0field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 11 citations in OpenAlex.

  1. Targeting Neuraminidase 4 Attenuates Kidney Fibrosis in Mice.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024
    Article
  2. Review
  3. Review
  4. Inhibitors of the Sialidase NEU3 as Potential Therapeutics for Fibrosis.International journal of molecular sciences · 2022
    Review
  5. Nefrologia : publicacion oficial de la Sociedad Espanola Nefrologia · 2022
    Review
  6. Article
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 4 institutions in 1 country.

Sang W HyunBaltimore Veterans Affairs Medical Center, Research Service, Baltimore, MD, 21201, USA.
Chiguang FengDepartment of Medicine, University of Maryland School of Medicine, HSF2 Room 303, 20 Penn St., Baltimore, MD, 21201, USA.
Anguo LiuBaltimore Veterans Affairs Medical Center, Research Service, Baltimore, MD, 21201, USA.
Erik P LillehojDepartment of Pediatrics, University of Maryland School of Medicine, Baltimore, MD, 21201, USA.
Rossana TrottaDepartment of Medicine, University of Maryland School of Medicine, HSF2 Room 303, 20 Penn St., Baltimore, MD, 21201, USA.
Tami J KingsburyDepartment of Physiology, University of Maryland School of Medicine, Baltimore, MD, 21201, USA.
Antonino PassanitiDepartment of Medicine, University of Maryland School of Medicine, HSF2 Room 303, 20 Penn St., Baltimore, MD, 21201, USA.
Katerina N LugkeyDepartment of Medicine, University of Maryland School of Medicine, HSF2 Room 303, 20 Penn St., Baltimore, MD, 21201, USA.
Sitara ChauhanDivision of Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD, 20993, USA.
John F CipolloDivision of Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD, 20993, USA.
Irina G LuzinaBaltimore Veterans Affairs Medical Center, Research Service, Baltimore, MD, 21201, USA.
Sergei P AtamasBaltimore Veterans Affairs Medical Center, Research Service, Baltimore, MD, 21201, USA.
Alan S CrossDepartment of Medicine, University of Maryland School of Medicine, HSF2 Room 303, 20 Penn St., Baltimore, MD, 21201, USA.
Simeon E GoldblumBaltimore Veterans Affairs Medical Center, Research Service, Baltimore, MD, 21201, USA. sgoldblu@som.umaryland.edu.
University of Maryland, Baltimore · USVeterans Health Administration · USCenter for Biologics Evaluation and Research · USUniversity of Maryland Marlene and Stewart Greenebaum Comprehensive Cancer Center

Funding

QAQC Johns Hopkins Institute for Clinical and Translational ResearchUL1TR003098 · NCATS · JOHNS HOPKINS UNIVERSITY · PI FORD, DANIEL ERNEST · 2019 to 2023
$57.7M
FDA HHS Z01 BJ 02044-13 LBPNCATS NIH HHS UL1 TR003098VA I01BX002352
6 · The paper itself

Abstract

To gain insight into sialic acid biology and sialidase/neuraminidase (NEU) expression in mature human neutrophil (PMN)s, we studied NEU activity and expression in PMNs and the HL60 promyelocytic leukemic cell line, and changes that might occur in PMNs undergoing apoptosis and HL60 cells during their differentiation into PMN-like cells. Mature human PMNs contained NEU activity and expressed NEU2, but not NEU1, the NEU1 chaperone, protective protein/cathepsin A(PPCA), NEU3, and NEU4 proteins. In proapoptotic PMNs, NEU2 protein expression increased > 30.0-fold. Granulocyte colony-stimulating factor protected against NEU2 protein upregulation, PMN surface desialylation and apoptosis. In response to 3 distinct differentiating agents, dimethylformamide, dimethylsulfoxide, and retinoic acid, total NEU activity in differentiated HL60 (dHL60) cells was dramatically reduced compared to that of nondifferentiated cells. With differentiation, NEU1 protein levels decreased > 85%, PPCA and NEU2 proteins increased > 12.0-fold, and 3.0-fold, respectively, NEU3 remained unchanged, and NEU4 increased 1.7-fold by day 3, and then returned to baseline. In dHL60 cells, lectin blotting revealed decreased α2,3-linked and increased α2,6-linked sialylation. dHL60 cells displayed increased adhesion to and migration across human bone marrow-derived endothelium and increased bacterial phagocytosis. Therefore, myeloid apoptosis and differentiation provoke changes in NEU catalytic activity and protein expression, surface sialylation, and functional responsiveness.

Indexed as

N-Acetylneuraminic AcidNeuraminidaseApoptosisCell DifferentiationHumansNeutrophilsN-Acetylneuraminic AcidNeuraminidase

Identifiers

PMID35986080
PMCPMC9390117
OpenAlexW4292377536

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.