Evidence map›Paper›PMID 35980589›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Preparation of Drosophila Tissues and Organs for Transmission Electron Microscopy.

Olympia-Ekaterini Psathaki, Achim Paululat

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Olympia-Ekaterini PsathakiUniversity of Osnabrück, Department of Zoology and Developmental Biology, Osnabrück, Germany.
Achim PaululatUniversity of Osnabrück, Department of Zoology and Developmental Biology, Osnabrück, Germany. achim.paululat@uos.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Transmission electron microscopy (TEM) is the method of choice to image the ultrastructure of cells or tissues. TEM allows the visualization of molecular complexes up to an atomic resolution. Thus, TEM data have led to important conclusions about cellular processes and supported findings obtained by functional analyses. In this chapter, we describe the preparation of Drosophila tissues for TEM and provide reliable step-by-step protocols for applying classical chemical fixation or high-pressure freezing-freeze substitution (HPF-FS) to preserve cellular structures.

Indexed as

CryopreservationDrosophilaAnimalsFreeze SubstitutionHistological TechniquesMicroscopy, Electron, TransmissionChemical fixationDrosophilaFreeze-substitutionHigh-pressure freezingTransmission electron microscopy

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.