Evidence map›Paper›PMID 35980530›Full record

ArticleMolecular biology reports2022

Construction of Fzd6

Xiaoru Yan, Junting Yang, Lanfei Xiao, Jiping Gao, Guoqiang Xu, Liang Wang, Yang Qi, Guohua Song

Abstract read
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In one paragraph

Article in Molecular biology reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Xiaoru Yan *School of Mental Health, Shanxi Medical University, South Shifang Street 55, Taiyuan, 030001, China.
Junting Yang *Laboratory Animal Center, Shanxi Key Laboratory of Experimental Animal Science and Human Disease Animal Model, Shanxi Medical University, Road Xinjian 56, Taiyuan, 030001, Shanxi, China.
Lanfei XiaoLaboratory Animal Center, Shanxi Key Laboratory of Experimental Animal Science and Human Disease Animal Model, Shanxi Medical University, Road Xinjian 56, Taiyuan, 030001, Shanxi, China.
Jiping GaoLaboratory Animal Center, Shanxi Key Laboratory of Experimental Animal Science and Human Disease Animal Model, Shanxi Medical University, Road Xinjian 56, Taiyuan, 030001, Shanxi, China.
Guoqiang XuLaboratory Animal Center, Shanxi Key Laboratory of Experimental Animal Science and Human Disease Animal Model, Shanxi Medical University, Road Xinjian 56, Taiyuan, 030001, Shanxi, China.
Liang WangSchool of Mental Health, Shanxi Medical University, South Shifang Street 55, Taiyuan, 030001, China.
Yang QiSchool of Mental Health, Shanxi Medical University, South Shifang Street 55, Taiyuan, 030001, China.
Guohua SongSchool of Mental Health, Shanxi Medical University, South Shifang Street 55, Taiyuan, 030001, China. ykdsgh@sxmu.edu.cn.ORCID http://orcid.org/0000-0002-0940-1252
Shanxi Medical University · CNShanxi Agricultural University · CN

Funding

Shanxi Provincial Health Commission 2020SYS10Shanxi Provincial Health Commission 2021031
6 · The paper itself

Abstract

backgroundThe CRISPR/Cas9 system is widely used for genome editing in human, rat and mouse cells. In this study, we established Fzd6 mutant mice using CRISPR/Cas9 technology, and obtained Fzd6 homozygous mutant (Fzd6 METHODS AND

resultsThe target of Fzd6 mutation was obtained by CRISPR/Cas9 technology and hippocampal tissue was collected for Nissl staining and histological analysis. Blood was collected for enzyme linked immunosorbent assay (ELISA); The gene expression of Fzd6 and the related genes expression in wnt pathway was quantified by quantitative real-time PCR (qRT-PCR), and then expression of Fzd6 and proteins in the Wnt pathway were identified by western blotting. ELISA results showed that the expression levels of brain derived neurotrophic factor (BDNF), 5-hydroxytryptamine (5-HT), and Noradrenaline (NE) in serum were significantly decreased in Fzd6

conclusionOur study successfully established homozygous Fzd6 mutant mice model. The relationship between Fzd6-Wnt and depression was preliminarily clarified, which provides an ideal animal model for subsequent research on diseases induced by the Fzd6 mutation.

Indexed as

Brain-Derived Neurotrophic FactorCRISPR-Cas SystemsAnimalsFrizzled ReceptorsGlycogen Synthase Kinase 3 betaHumansMiceNorepinephrineRatsReproductionRNA, MessengerSerotoninTechnologyBrain-Derived Neurotrophic FactorFrizzled ReceptorsFZD6 protein, humanGlycogen Synthase Kinase 3 betaNorepinephrineRNA, MessengerSerotoninCRISPR/Cas9Fzd6Gene mutationGenotype identificationWnt signaling pathway

Identifiers

PMID35980530
OpenAlexW4292405811

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.