Evidence map›Paper›PMID 35979174›Full record

ArticleJournal of orthopaedic translation2022

Differential dynamics of bone graft transplantation and mesenchymal stem cell therapy during bone defect healing in a murine critical size defect.

Elijah Ejun Huang, Ning Zhang, Edward A Ganio, Huaishuang Shen, Xueping Li, Masaya Ueno, Takeshi Utsunomiya, Masahiro Maruyama, Qi Gao, Ni Su and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of orthopaedic translation, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
2.4field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 16 citations in OpenAlex.

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  11. Recent advances of responsive scaffolds in bone tissue engineering.Frontiers in bioengineering and biotechnology · 2023
    Review
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 2 institutions in 1 country.

Elijah Ejun HuangDepartment of Orthopaedic Surgery, Stanford University, Stanford, CA, USA.
Ning ZhangDepartment of Orthopaedic Surgery, Stanford University, Stanford, CA, USA.
Edward A GanioDepartment of Anesthesiology, Perioperative and Pain Medicine, Stanford University, Stanford, CA, USA.
Huaishuang ShenDepartment of Orthopaedic Surgery, Stanford University, Stanford, CA, USA.
Xueping LiDepartment of Orthopaedic Surgery, Stanford University, Stanford, CA, USA.
Masaya UenoDepartment of Orthopaedic Surgery, Stanford University, Stanford, CA, USA.
Takeshi UtsunomiyaDepartment of Orthopaedic Surgery, Stanford University, Stanford, CA, USA.
Masahiro MaruyamaDepartment of Orthopaedic Surgery, Stanford University, Stanford, CA, USA.
Qi GaoDepartment of Orthopaedic Surgery, Stanford University, Stanford, CA, USA.
Ni SuDepartment of Orthopaedic Surgery, Stanford University, Stanford, CA, USA.
Zhenyu YaoDepartment of Orthopaedic Surgery, Stanford University, Stanford, CA, USA.
Fan YangDepartment of Orthopaedic Surgery, Stanford University, Stanford, CA, USA.
Brice GaudillièreDepartment of Anesthesiology, Perioperative and Pain Medicine, Stanford University, Stanford, CA, USA.
Stuart B GoodmanDepartment of Orthopaedic Surgery, Stanford University, Stanford, CA, USA.
Stanford University · USStanford Medicine · US

Funding

Harnessing the human monocyte system to improve surgical recoveryR35GM137936 · NIGMS · STANFORD UNIVERSITY · PI GAUDILLIERE, BRICE · 2020 to 2024
$2.0M
NIGMS NIH HHS R35 GM137936
6 · The paper itself

Abstract

Background: A critical size bone defect is a clinical scenario in which bone is lost or excised due to trauma, infection, tumor, or other causes, and cannot completely heal spontaneously. The most common treatment for this condition is autologous bone grafting to the defect site. However, autologous bone graft is often insufficient in quantity or quality for transplantation to these large defects. Recently, tissue engineering methods using mesenchymal stem cells (MSCs) have been proposed as an alternative treatment. However, the underlying biological principles and optimal techniques for tissue regeneration of bone using stem cell therapy have not been completely elucidated. Methods: In this study, we compare the early cellular dynamics of healing between bone graft transplantation and MSC therapy in a murine chronic femoral critical-size bone defect. We employ high-dimensional mass cytometry to provide a comprehensive view of the differences in cell composition, stem cell functionality, and immunomodulatory activity between these two treatment methods one week after transplantation. Results: We reveal distinct cell compositions among tissues from bone defect sites compared with original bone graft, show active recruitment of MSCs to the bone defect sites, and demonstrate the phenotypic diversity of macrophages and T cells in each group that may affect the clinical outcome. Conclusion: Our results provide critical data and future directions on the use of MSCs for treating critical size defects to regenerate bone.

Indexed as

Bone graftCritical-size bone defectCyTOFMacrophagesStem cellsT cells

Identifiers

PMID35979174
PMCPMC9357712
OpenAlexW4289841277

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.