ArticleBMC musculoskeletal disorders2022
miR-215-5p regulates osteoporosis development and osteogenic differentiation by targeting XIAP.
Article in BMC musculoskeletal disorders, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 14 citations in OpenAlex.
- miR-613 regulates osteogenic differentiation by targeting TIMP3 in postmenopausal osteoporosis.Hormones (Athens, Greece) · 2026Article
- Adipose-derived stem cell exosomes mitigate ferroptosis and enhance osteogenesis via miR-215-5p-mediated ubiquitin-specific protease 1 suppression.Endocrine journal · 2026Article
- Mechanisms underlying the role of TNF-α and IL-1β preconditioned exosomes derived from human umbilical cord mesenchymal stem cells in wound healing.Frontiers in immunology · 2026Article
- LncRNA LINC01123-mediated impaired fracture healing through miR-134-5p/XIAP axis.Journal of orthopaedic surgery and research · 2025Article
- miR-127-3p regulates osteoporosis progression by targeting VAMP2 to modulate cell differentiation.The Journal of endocrinology · 2025Article
- MiR-23a-5p affects postmenopausal osteoporosis by targeting ALX3 to regulate osteoblast differentiation.Journal of orthopaedic surgery and research · 2025Article
- Circ_0003072 Mediates the Pro-osteogenic Differentiation Effect of Betulinic Acid on Human Periodontal Ligament Stem Cells.International dental journal · 2025Article
- miR-468-3p suppresses osteogenic differentiation of BMSCs by targeting Runx2 and inhibits bone formation.Journal of orthopaedic surgery and research · 2024Article
- Role of mir-32-3p in the diagnosis and risk assessment of osteoporotic fractures.Journal of orthopaedic surgery and research · 2024Article
- Exploring the effects of naringin on oxidative stress-impaired osteogenic differentiation via the Wnt/β-catenin and PI3K/Akt pathways.Scientific reports · 2024Article
- Common miRNAs of Osteoporosis and Fibromyalgia: A Review.International journal of molecular sciences · 2023Review
- Sharing Circulating Micro-RNAs between Osteoporosis and Sarcopenia: A Systematic Review.Life (Basel, Switzerland) · 2023Review
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOsteoporosis (OP) is a metabolic disease that involves microstructure destruction and fracture damage. The present study probed into the significance of miR-215-5p in OP progression.
methodsSerum samples were collected from surgical patients and healthy controls. qRT-PCR analysis was utilized to determine the miR-215-5p level in clinical samples and human bone mesenchymal stem cells (hBMSCs) induced by β-glycerol phosphate. A dual luciferase reporter assay was exploited to examine the targeted relationship between miR-215-5p and XIAP. The mineralization and calcium deposition of hBMSCs were assessed by detection of ALP activity, Alizarin red staining, and osteoblast marker expression. Protein expression was determined by western blot analysis.
resultsMiR-215-5p was significantly reduced in patients with OP and increased in hBMSCs treated with β-glycerophosphate. Enhanced miR-215-5p level triggered augment in osteoblast markers (Alkaline phosphatase/ ALP, Osteocalcin/ OCN, and Runt-Related Transcription Factor 2/ Runx2), which was accompanied by the increase of ALP activity in hBMSCs and accumulation of Calcium. Functional experiments show that XIAP was a target of miR-215-5p and negatively modulated by miR-215-5p. XIAP expression levels were increased in OP samples, and decreased XIAP in β-glycerophosphate-treated hBMSCs inhibited its' osteogenic differentiation. Functional loss and acquisition experiments depicted that miR-215-5p promoted the differentiation of hBMSCs by inhibiting the XIAP level, playing a protective role in the pathogenesis of OP.
conclusionsβ-glycerophosphate promoted the osteogenic differentiation of hBMSCs, increased miR-215-5p level, and decreased XIAP. miR-215-5p stimulated osteogenic differentiation of hBMSCs by targeting XIAP, shedding new insights for the detection and therapy of OP.
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