Evidence map›Paper›PMID 35978294›Full record

ArticleBMC infectious diseases2022

Molecular characterization of multidrug-resistant ESKAPEE pathogens from clinical samples in Chonburi, Thailand (2017-2018).

Sirigade Ruekit, Apichai Srijan, Oralak Serichantalergs, Katie R Margulieux, Patrick Mc Gann, Emma G Mills, William C Stribling, Theerasak Pimsawat, Rosarin Kormanee, Suthisak Nakornchai and 7 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC infectious diseases, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 5 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed, 5 pooled it
3.4field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 5 syntheses or guidelines pooled it, 53 citations in OpenAlex.

  1. Genetic Fingerprint ofPathogens (Basel, Switzerland) · 2026
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  13. The Co-Existence ofAntibiotics (Basel, Switzerland) · 2025
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  14. Comprehensive Genomic Analysis ofAntibiotics (Basel, Switzerland) · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors at 4 institutions in 2 countries.

Sirigade RuekitBacterial and Parasitic Diseases Department, Armed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok, 10400, Thailand.
Apichai SrijanBacterial and Parasitic Diseases Department, Armed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok, 10400, Thailand.
Oralak SerichantalergsBacterial and Parasitic Diseases Department, Armed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok, 10400, Thailand.
Katie R MargulieuxWound Infections Department, Bacterial Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Patrick Mc GannMultidrug-Resistant Organism Repository and Surveillance Network (MRSN), Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Emma G MillsMultidrug-Resistant Organism Repository and Surveillance Network (MRSN), Walter Reed Army Institute of Research, Silver Spring, MD, USA.
William C StriblingMultidrug-Resistant Organism Repository and Surveillance Network (MRSN), Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Theerasak PimsawatQueen Sirikit Naval Hospital, Chonburi, Thailand.
Rosarin KormaneeQueen Sirikit Naval Hospital, Chonburi, Thailand.
Suthisak NakornchaiQueen Sirikit Naval Hospital, Chonburi, Thailand.
Chaiwat SakdinavaQueen Sirikit Naval Hospital, Chonburi, Thailand.
Prawet SukhchatQueen Sirikit Naval Hospital, Chonburi, Thailand.
Mariusz WojnarskiBacterial and Parasitic Diseases Department, Armed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok, 10400, Thailand.
Samandra T DemonsBacterial and Parasitic Diseases Department, Armed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok, 10400, Thailand.
John M CrawfordUS Army Medical Research Institute of Chemical Defense, Gunpowder, MD, USA.
Paphavee LertsethtakarnBacterial and Parasitic Diseases Department, Armed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok, 10400, Thailand. PaphaveeL@afrims.org.
Brett E SwierczewskiBacterial Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Armed Forces Research Institute of Medical Science · THChonburi Hospital · THWalter Reed Army Institute of Research · USUnited States Army Medical Research Institute of Infectious Diseases · US

Funding

Armed Forces Health Surveillance Division, Global Emerging Infections Surveillance Branch (AFHSD-GEIS) ProMID ID P0076_17_AF
6 · The paper itself

Abstract

backgroundESKAPEE pathogens Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter spp. and Escherichia coli are multi-drug resistant (MDR) bacteria that present increasing treatment challenges for healthcare institutions and public health worldwide.

methods431 MDR ESKAPEE pathogens were collected from Queen Sirikit Naval Hospital, Chonburi, Thailand between 2017 and 2018. Species identification and antimicrobial resistance (AMR) phenotype were determined following CLSI and EUCAST guidelines on the BD Phoenix System. Molecular identification of antibiotic resistant genes was performed by polymerase chain reaction (PCR), real-time PCR assays, and whole genome sequencing (WGS).

resultsOf the 431 MDR isolates collected, 1.2% were E. faecium, 5.8% were S. aureus, 23.7% were K. pneumoniae, 22.5% were A. baumannii, 4.6% were P. aeruginosa, 0.9% were Enterobacter spp., and 41.3% were E. coli. Of the 401 Gram-negative MDR isolates, 51% were carbapenem resistant, 45% were ESBL producers only, 2% were colistin resistance and ESBLs producers (2%), and 2% were non-ESBLs producers. The most prevalent carbapenemase genes were bla

conclusionsThis report highlights the prevalence of AMR among clinical ESKAPEE pathogens in eastern Thailand. E. coli was the most common MDR pathogen collected, followed by K. pneumoniae, and A. baumannii. Carbapenem-resistant Enterobacteriaceae (CRE) and extended spectrum beta-lactamases (ESBLs) producers were the most common resistance profiles. The co-occurrence of mcr-1 and mcr-3 in 2 E. coli strains, which did not affect the level of colistin resistance, is also reported. The participation of global stakeholders and surveillance of MDR remain essential for the control and management of MDR ESKAPEE pathogens.

Indexed as

ColistinEscherichia coli ProteinsAnti-Bacterial Agentsbeta-LactamasesDrug Resistance, Multiple, BacterialEscherichia coliKlebsiella pneumoniaeMicrobial Sensitivity TestsPseudomonas aeruginosaStaphylococcus aureusThailandAnti-Bacterial Agentsbeta-LactamasesColistinEscherichia coli ProteinsMCR-1 protein, E coliCREESBLESKAPEEmcrMDRThailand

Identifiers

PMID35978294
PMCPMC9386987
OpenAlexW4292092827

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.