Evidence map›Paper›PMID 35978099›Full record

ArticleJournal of human hypertension2023

Urinary extracellular vesicle mRNA analysis of sodium chloride cotransporter in hypertensive patients under different conditions.

Francesca Pizzolo, Lorenzo Bertolone, Annalisa Castagna, Francesca Morandini, Giulia Sartori, Domenica De Santis, Natalia Tiberti, Paolo Brazzarola, GianLuca Salvagno, Simonetta Friso and 1 more

Abstract read
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In one paragraph

Article in Journal of human hypertension, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 2 countries.

Francesca PizzoloDepartment of Medicine, Unit of Internal medicine, University of Verona, Verona, Italy. francesca.pizzolo@univr.it.ORCID http://orcid.org/0000-0003-4419-3994
Lorenzo BertoloneDepartment of Medicine, Unit of Internal medicine, University of Verona, Verona, Italy.
Annalisa CastagnaDepartment of Medicine, Unit of Internal medicine, University of Verona, Verona, Italy.
Francesca MorandiniDepartment of Medicine, Unit of Internal medicine, University of Verona, Verona, Italy.
Giulia SartoriDepartment of Medicine, Unit of Internal medicine, University of Verona, Verona, Italy.
Domenica De SantisDepartment of Medicine, Unit of Internal medicine, University of Verona, Verona, Italy.ORCID http://orcid.org/0000-0002-8966-4377
Natalia TibertiDepartment of Infectious, Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital, Negrar, Italy.
Paolo BrazzarolaDepartment of General Surgery and Pancreatic Surgery, "G. B. Rossi" University Hospital, Verona, Italy.
GianLuca SalvagnoSection of Clinical Biochemistry, University and Azienda Ospedaliera Universitaria Integrata of Verona, Verona, Italy.
Simonetta FrisoDepartment of Medicine, Unit of Internal medicine, University of Verona, Verona, Italy.ORCID http://orcid.org/0000-0002-9606-6711
Oliviero OlivieriDepartment of Medicine, Unit of Internal medicine, University of Verona, Verona, Italy.ORCID http://orcid.org/0000-0001-8209-9056
University of Verona · ITAzienda Ospedaliera Universitaria Integrata Verona · ITOspedale Sacro Cuore Don Calabria · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Urinary extracellular vesicles (UEV) mainly derive from cells of the urogenital tract and their cargo (proteins, nucleic acids, lipids, etc.) reflects their cells of origin. Na chloride cotransporter (NCC) is expressed at the kidney level in the distal convoluted tubule, is involved in salt reabsorption, and is the target of the diuretic thiazides. NCC protein has been recognized and quantified in UEV in previous studies; however, UEV NCC mRNA has never been studied. This study aimed to identify and analyze NCC mRNA levels in primary aldosteronism (PA). The rationale for this investigation stems from previous observations regarding NCC (protein) as a possible biomarker for the diagnosis of PA. To evaluate modulations in the expression of NCC, we analyzed NCC mRNA levels in UEV in PA and essential hypertensive (EH) patients under different conditions, that is, before and after saline infusion, anti-aldosterone pharmacological treatment, and adrenal surgery. NCC mRNA was measured by RT-qPCR in all the samples and was regulated by volume expansion. Its response to mineralocorticoid receptor antagonist was correlated with renin, and it was increased in PA patients after adrenalectomy. NCC mRNA is evaluable in UEV and it can provide insights into the pathophysiology of distal convolute tubule in different clinical conditions including PA.

Indexed as

Extracellular VesiclesHypertensionHumansKidney Tubules, DistalRNA, MessengerSodiumSodium Chloride SymportersRNA, MessengerSodiumSodium Chloride Symporters

Identifiers

PMID35978099
OpenAlexW4292259493

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.