Evidence map›Paper›PMID 35977472›Full record

ArticleCell reports. Medicine2022

A single-cell transcriptional gradient in human cutaneous memory T cells restricts Th17/Tc17 identity.

Christopher P Cook, Mark Taylor, Yale Liu, Ralf Schmidt, Andrew Sedgewick, Esther Kim, Ashley Hailer, Jeffrey P North, Paymann Harirchian, Hao Wang and 10 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports. Medicine, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.7field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 20 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Review
  6. Article
  7. Review
  8. Article
  9. Article
  10. From PsO to PsA: the role of TFrontiers in medicine · 2024
    Review
  11. Post-transcriptional checkpoints in autoimmunity.Nature reviews. Rheumatology · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors at 8 institutions in 3 countries.

Christopher P CookDepartment of Dermatology, University of California, San Francisco, San Francisco, CA, USA; Dermatology, Veterans Affairs Medical Center, San Francisco, CA, USA.
Mark TaylorDepartment of Dermatology, University of California, San Francisco, San Francisco, CA, USA; Clinical Research Centre, Medical University of Białystok, Białystok, Poland.
Yale LiuDermatology, Veterans Affairs Medical Center, San Francisco, CA, USA; Department of Dermatology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, ShaanXi 710004, P.R. China.
Ralf SchmidtGladstone-UCSF Institute of Genomic Immunology, San Francisco, CA, USA.
Andrew SedgewickNantHealth, Inc, El Segundo, CA, USA.
Esther KimDivision of Plastic Surgery, University of California, San Francisco, San Francisco, CA, USA.
Ashley HailerDepartment of Dermatology, University of California, San Francisco, San Francisco, CA, USA.
Jeffrey P NorthDepartment of Dermatology, University of California, San Francisco, San Francisco, CA, USA.
Paymann HarirchianDepartment of Dermatology, University of California, San Francisco, San Francisco, CA, USA; Dermatology, Veterans Affairs Medical Center, San Francisco, CA, USA.
Hao WangDepartment of Statistics, University of California, Berkeley, Berkeley, CA, USA.
Sakeen W KashemDepartment of Dermatology, University of California, San Francisco, San Francisco, CA, USA; Dermatology, Veterans Affairs Medical Center, San Francisco, CA, USA.
Yanhong ShouDepartment of Dermatology, Huashan Hospital, Fudan University, Shanghai, P.R. China.
Timothy C McCalmontDepartment of Dermatology, University of California, San Francisco, San Francisco, CA, USA; Department of Pathology, University of California, San Francisco, San Francisco, CA, USA; Golden State Dermatology Associates, Walnut Creek, CA, USA.
Stephen C BenzNantHealth, Inc, El Segundo, CA, USA.
Jaehyuk ChoiDepartment of Dermatology, Northwestern University, Evanston, IL, USA.
Elizabeth PurdomDepartment of Statistics, University of California, Berkeley, Berkeley, CA, USA.
Alexander MarsonGladstone-UCSF Institute of Genomic Immunology, San Francisco, CA, USA.
Silvia B V RamosDepartment of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Jeffrey B ChengDepartment of Dermatology, University of California, San Francisco, San Francisco, CA, USA; Dermatology, Veterans Affairs Medical Center, San Francisco, CA, USA. Electronic address: Jeffrey.Cheng@ucsf.edu.
Raymond J ChoDepartment of Dermatology, University of California, San Francisco, San Francisco, CA, USA. Electronic address: raymond.cho@ucsf.edu.
San Francisco VA Medical Center · USUniversity of California, San Francisco · USGladstone Institutes · USUniversity of California, Berkeley · USFudan University · CNMedical University of Białystok · PLNorthwestern University · USUniversity of North Carolina at Chapel Hill · US

Funding

Non-coding RNA structure change in Chronic Obstructive Pulmonary DiseaseR01HL111527 · NHLBI · UNIV OF NORTH CAROLINA CHAPEL HILL · PI Alain T Laederach, Kevin M Weeks · 2012 to 2026
$6.6M
UCSF Dermatology Training GrantT32AR007175 · NIAMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Wilson Liao, Michael David Rosenblum · 1986 to 2026
$6.1M
Role of DNA Methylation and Hydroxymethylation in Epidermal HomeostasisK08AR067243 · NIAMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI CHENG, JEFFREY B · 2015 to 2019
$834k
NHLBI NIH HHS R01 HL111527NIAMS NIH HHS K08 AR067243
6 · The paper itself

Abstract

The homeostatic mechanisms that fail to restrain chronic tissue inflammation in diseases, such as psoriasis vulgaris, remain incompletely understood. We profiled transcriptomes and epitopes of single psoriatic and normal skin-resident T cells, revealing a gradated transcriptional program of coordinately regulated inflammation-suppressive genes. This program, which is sharply suppressed in lesional skin, strikingly restricts Th17/Tc17 cytokine and other inflammatory mediators on the single-cell level. CRISPR-based deactivation of two core components of this inflammation-suppressive program, ZFP36L2 and ZFP36, replicates the interleukin-17A (IL-17A), granulocyte macrophage-colony-stimulating factor (GM-CSF), and interferon gamma (IFNγ) elevation in psoriatic memory T cells deficient in these transcripts, functionally validating their influence. Combinatoric expression analysis indicates the suppression of specific inflammatory mediators by individual program members. Finally, we find that therapeutic IL-23 blockade reduces Th17/Tc17 cell frequency in lesional skin but fails to normalize this inflammatory-suppressive program, suggesting how treated lesions may be primed for recurrence after withdrawal of treatment.

Indexed as

Memory T CellsTh17 CellsHumansInflammationInflammation MediatorsSkinInflammation Mediatorscytokineinflammationpsoriasisresident-memory T celltristetraprolin scRNA-seqZFP36ZFP36L2

Identifiers

PMID35977472
PMCPMC9418858
OpenAlexW4292055498

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.